Compare the size of cheek cell and onion cell DCP: Introduction: In this experiment, Onion cells (Allium cepa) Cheek cells (Homo sapiens) were measured and compared. The aim of this experiment is to find out how both cells differ by comparing their size. Onion cells (Allium cepa) is plant cell and Cheek cells (Homo sapiens) is an animal cell. Hypothesis: The size of an animal cell (Homo sapiens) should be smaller than a plant cell (Allium cepa). Because a plant cell has cell wall which is to maintain the size of it while animal cell doesn’t.
Also, plant cell usually has a large vacuole and chloroplast, therefore it should be larger than an animal cell. The estimate size of plant cell is between 300µm too 500µm1, animal cell should be around 1-100µm 2. Method: 1. Measuring the ratio of the microscope First, set up a microscope on the 40x objective and focus it on the ‘small ruler’. Then, adjusted the microscope stage till the ‘small ruler’ touches the left and the right-most point.
Record it can calculate the ratio. 2. Preparing the slides of cells
Peel a piece of onion then place it on a eye piece. Add one drop of iodine solution then place a cover slip on top of it. Use a Q- tip to collect cheek cell from your cheek. Rub it against the eye piece, add a drop of methylene blue then cover it with a cover slip. Make sure that there’s no air bubble in it. 3. Find the size of each cell Measure the length of 20 onion cells and 20 cheek cells on the 40x objective. Estimate the size of the cell by fitting them into the ruler from your eye piece. Apparatus: Microscope (x40) -Eye piece -Onion -Microscope slide x2 -Cover slip x2 -Forceps Magnification: 100 division = 1µm 1 division = 10µm ±0. 5 mm Result: Measured length of Onion cell and Cheek cell Measured length of Onion cell /µm (±10)Measured length of cheek cell /µm (±10) 50070 50060 25050 37040 27060 29050 50030 46040 28020 36070 50050 34070 30080 60050 28060 48030 43020 35060 23020 35040 382±106. 31µm48. 5±18. 14µm Data Processing: T-test: Mean of the sample: Group1: 382 Group2: 48. 5 Hypothesis :
HO: The size of cheek cell(Homo sapiens)and onion cell (Allium cepa) are similar HA: The size of cheek cell(Homo sapiens)and onion cell (Allium cepa) are not similar Unpaired 2-tailed t-test Df: 38 P-Value: 2. 06983E-16 Qualitative observation: Onion cell(Allium cepa): – appeared as colourless -A drop of iodine was added, onion cell (Allium cepa) turned yellowish orange -Rectangular shape -Bigger than cheek cell (Homo sapiens) Cheek cell (Homo sapiens): -Appeared as colourless -A drop of methylene blue was added, cheek cell (Homo sapiens) turned blue. Rounded in shape -Smaller than onion cell (Allium cepa) CE Conclusion: After all the investigation, it proves that plant cell has a larger size than animal cell. As the graph show, the sizes of Homo sapiens are quite regular while Allium cepa have quite extreme size. The smallest size of Allium cepa is 230µm and the biggest is 600µm their difference is 370µm. Homo sapiens has a smaller difference which is 60µm. Comparing to the literature value, onion cell should have a size between 300µm to 500µm. The average size of 300µm to 500µm. The average size of Allium cepa is 382 Onion cell used was fresh so that the result is more likely between the range.. However, there were some Allium cepa appeared to be larger than 500µm. The size of Homo sapiens is 48. 5 and the literature value is 30. 1 Some of the cheek cell seems to be a little bit bigger because before the experiment, the cheek cell donor drank some water. Due to osmosis, cheek cell became turgid therefore size of it increase. P value was less than 0. 05, therefore null hypothesis is rejected. Mean values are significantly different.
Evaluation: It’s difficult to really see the length of the cell. Since the cells are really small. In this case, we can use a larger magnification for example use 100x rather than 40x. Also, we may measure the same cell over and over again since all cells looks alike. We can divide the slide into different sections, try not to go back to the same area again. Lastly, the size of the cheek cell maybe affected by water potential since there’s saliva in our mouth. Try not to drink water for a certain of time in order to keep it as a fair test.
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