Fluorescence study of dyes Essay
Fluorescence Study of theKinetics of Energy Transfer Between Dyes1. IntroductionCoumarin 1 and Sodium fluorescein are two dyes which absorb and emit light in the visible region. By using a spectrophotometer we are recording an absorption spectrum and then determining the molar decadic absorption coefficients, which will be used later to interpret and analyse the fluorescence spectra.
For fluorescence spectra, there are two kinds, the excitation and the emission spectrum. The excitation spectrum is obtained by measuring the intensity of the emission as the excitation wavelength is altered by scanning a monochromator.
The emission spectrum is obtained by measuring the emission intensity as a function of wavelength for excitation at a fixed wavelength.
An absorption spectrum and an excitation spectrum are by their nature actually equivalent.
The two dyes exhibit energy transfer properties. The donor is coumarin and the acceptor the fluorescein. When the donor is excited, it is naturally decaying to the ground state. However in presence of an acceptor, this process is enhanced trough energy transfer.
We will distinguish between collisional energy transfer and dipole-dipole transfer. From here we will try to determine the quenching constant and the distance at which decay and energy transfer are equally probable, as well as prove that Stern-Volmer’s law and Frster’s theory are obeyed.
2. Results2.1. Electronic Absorption SpectraWe made up stock solutions for both salts and diluted them down to use in the UV/vis spectrometer.
Coumarin 1C = 8 x10-5 mol dm -3We obtained a spectrum with a maximum at 376.5nm at an absorbance of 1.4573, using Beer-Lambert’s law, we deduced the molar decadic absorption coefficients.
Sodium fluoresceinC = 2.425 x10-5 mol dm -3Here max was 500.5 nm at an absorbance of 2.0922Summary of the results obtained.
Dye max (nm)max (m2 mol-1) (m2 mol-1)’ (m2 mol-1)D (Coumarin)376.51.82 x 109—1.25 x 106A (Fluorescein)500.55.17 x 1071.41 x 1068 x 1062.2. Fluorimetrya) Perylene standard/nmIEmission Spectrum4384.063EX = 434nm4672.620Excitation Spectrum4102.755EX = 438nm4374.208These are the values for the maxima in both spectrum, for the graphs, see attached sheet.
b) Coumarin 1 C = 4 x10-6 mol dm -3/nmIEmission Spectrum3741.050EX = 377nm4434.081Excitation Spectrum3734.156EX = 443 nm4461.026b) Sodium fluorescein C = 1.212 x10-6 mol dm -3/nmIEmission Spectrum5167.453EX = 501nmExcitation Spectrum5017.516EX = 516 nmOn the graphs of the standard and the two dyes, we can nicely see that the excitation and emission spectra are mirror images of each other overlaid. The excitation wavelength in one is the highest emitting one in the other.
2.3. Energy TransferStern-Volmer equation 0 /= 1 + K A(1)with 0 /being the ratio of quantum yield andK being the Stern-Volmer quenching constanta) Experimental studyTo test the Stern-Volmer equation, as well as to prove the dipole-dipole transfer, we did fluorimetric measurements with mixtures of different concentrations of D and A.
D AImratio0.003 070.238 1.001188 0.9883 0.003 4.042E-04 49.052 1.002212 1.4288 0.003 8.084E-04 38.766 1.003237 1.806 0.003 1.213E-03 31.416 1.004262 2.2263 0.003 1.617E-03 25.816 1.005285 2.7064 Table 1: Results from D-A mixtures experiments.
withIbeing the intensity of light emittedm being a geometric correction factorand ratio the ratio of the quantum yieldsThose values were used to fit the model expression (1),using the following program* NonLinear Regression.
MODEL PROGRAM K=1000000 .
COMPUTE PRED_ = 1+K*conc.
NLR ratio /OUTFILE=’C:win95TEMPSPSSFNLR.TMP’ /PRED PRED_ /SAVE PRED RESID /CRITERIA SSCONVERGENCE 1E-8 PCON 1E-8 .
Non-linear RegressionIteration Residual SSK1.0089160488 1000000.001.1.0031167306 1034392.202.0031167306 1034392.202.1.0031167306 1034392.20Run stopped after 4 model evaluations and 2 derivative evaluations.
Iterations have been stopped because the relative difference betweensuccessive parameter estimates is at most PCON = 1.000E-08Nonlinear Regression Summary StatisticsDependent Variable RATIO SourceDF Sum of Squares Mean Square Regression 1 18.55731 18.55731 Residual43.116731E-037.791826E-04 Uncorrected Total 5 18.56043 (Corrected Total) 4 1.79521 R squared = 1 – Residual SS / Corrected SS =.99826Asymptotic 95 % AsymptoticConfidence Interval ParameterEstimateStd. ErrorLowerUpper K1034392.1957 12606.399781 999391.21871 1069393.1726The crosses are marking the values obtained and the line is the fit based on (1).
So the best fit value for K is 1.034×106, with at confidence limit of +/- 3.5×104.
So K , the Stern-Volmer quenching constant is 1.034×103 mol-1 dm 3.
Parent variance 2ratio = 2.43×10-4.
The mean square residual s2ratio is 7.79×10-4Reduced chi-square 2v = 3.20. A value under 3 indicates a good fit, hence this shows that our data, is not really fitted onto the model.
b) Energy transfer rate constantFor coumarin, =0.64Its intrinsic fluorescence lifetime is 0s = 1/(1.822×108)Thereforektots = 1/ (0s)=2.846 s-1andket = K ktots= 2.943x1011mol-1 dm 3 s-1This value as an error estimation of +/- 9.961 x109mol-1 dm 3 s-1And as ket kdiff , we can see that 98% of the total energy transfer is due to dipole-dipole transfer.
c) Frster energy transferA1/2 = 9.67 x10-4 mol dm -3Transformation of the raw data through SPSS gives us the following graph.
Values obtained:JDA = 6.897×1031 nm mol -1R0 = 0.14 nm(R0)eff = 0.584 nm based on equation (A3-13)So we see that those are very clearly quite different, and that could show that it does not obey to Forster’s theory.
3. ConclusionSo we have shown that the quenching of Coumarin 1 by Sodium fluorescein is obeyed by Stern-Volmer kinetics, that there is mainly dipole-dipole transfer, but could not agree with Forster’s theory.