My progress Essay

In June, the main focus was to familiarize myself with the background for the project - My progress Essay introduction. This required an extensive literature search covering the role of IL-25 in allergic airway inflammation, and the relationship between mitogen-activated protein kinases (MAPKs) and asthma. At the same time, I also started to get familiar with the isolation procedure for B cells and other techniques that I might use in the project.

Based on literature review, the concentration of IL-25 used to achieve stimulation was in the broad range of 50ng/ml to 2µg/ml. After consulting my supervisor, I decided to first use 100ng/ml of IL-25 to stimulate the signalling pathway in stimulated B cells. Then the time-response of MAPKs (including P38 and P44/42) to IL-25 experiment was set up. The time intervals for p38 were 0, 5, 15, 30 minutes and one hour and the time intervals for p44/42 were 0, 1, 5, 15 and 30 minutes respectively. Flow cytometry was used to measure the MAPKs activation using phosphor-specific antibodies to p38 and p44/42. No phosphorated p38 was detected while a slight but not increase was detected for phosphated p44/42. For the second trial, the concentration of IL-25 was same as before, but I set up a positive control by using 10-8M PDB to stimulate MAPKs activation. The time intervals for this trial were 2 minutes and 5 minutes. A significant increase in the level of phophorated p44/42 was observed, but there was no change for IL-25 stimulation.

We will write a custom essay sample on
My progress Essay
or any similar topic specifically for you
Do Not Waste
Your Time
SEND

By clicking "SEND", you agree to our terms of service and privacy policy. We'll occasionally send you account related and promo emails.

More Essay Examples on Education Rubric

Since the two trials did not work well, my supervisor advised me to use CD23 (the marker on the B cells, can serve as a low affinity receptor for IgE and involved in the regulation of  IgE synthesis, and  the CD23 suspected to down regulate IL-25 ) to determine the function of IL-25 in B cells. This set up involved dose-response studies of IL-25, so B cells were incubated for 24 hours with100ng/ml and 200ng/ml of IL-25. A significant change was not observed after staining the cells with anti-human CD23. I think the experiment failed because of my lack of experience in flow cytometry and staining procedures, so I will repeat this experiment again to get better results.

Since fresh human B cell preparation involves long time and considering my project timeline, I will use RAMOS B cell line to repeat the above mentioned experiment.

Haven’t Found A Paper?

Let us create the best one for you! What is your topic?

By clicking "SEND", you agree to our terms of service and privacy policy. We'll occasionally send you account related and promo emails.

Haven't found the Essay You Want?

Get your custom essay sample

For Only $13.90/page

Eric from Graduateway Hi there, would you like to get an essay? What is your topic? Let me help you

logo