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Second Most Abundant Polysaccharide In Nature Biology

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Chitin is 2nd most abundant polyose in nature after cellulose. Chitin is a high molecular weight additive polymer. It is a copolymer of N-acetyl-glucosamine and N-glucosamine units linked by I?-D ( 1i? 4 ) bonds. If the figure of N-acetyl-glucosamine is higher than 50 % , it is known as Chitin. On the other manus if N-glucosamine units are higher than they are known as Chitosan ( Figure 1 ) .

Figure1. Conventional representation of the chitin and Chitosan picturing the co-polymer character of the biopolymers

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It resembles cellulose in solubility, as it is extremely indissoluble stuff with low chemical responsiveness.

It functions as a structural polyose of course. Chitin is a white, difficult, inelastic stuff and it causes batch of problem in coastal countries because it leads to come up pollution. Nitrogen content in Chitosan is high.i.e. 6.89 % as compared to synthetically substituted cellulose ( 1.25 % ) which makes due to this it is of great involvement commercially. Figure 2 shows the construction of cellulose, chitin and Chitosan.

Figure 2 Structures of cellulose, chitin, and Chitosan

Background information

Wound healing is a complicated procedure.

In instances of deformation of tissue construction, tissues may non be healed as it was earlier. Furthermore, it can take to hempen visual aspect take downing mechanical strength and which leads to mark formation. To get the better of this issue, biomaterials which provides better healing of tissues and avoiding cicatrix tissue formation plays critical function. Chitin, Chitosan have been found to advance wound mending peculiarly in stage of proliferation and matrix formation.

Problems faced in lesion dressing:

Major job faced in lesion healing includes antibiotic opposition of micro-organisms which causes infection and delayed lesion mending. In this article, research workers have developed fresh chitin /nanosilver composite scaffolds for lesion healing applications.

Why Ag is used:

Due to its polycationic nature Ag acts as an effectual antibacterial agent. It can be used in wound dressings as it is really effectual when used in signifiers of nanoparticles as the surface country to volume ratio of Ag nanoparticles is high which leads to better contact with bacteriums.

Antibacterial activity surveies:

Bacterial efficiency of Chitin/ nanosilver composite scaffolds against gm positive S.aureus is shown in figure 6a while gram negative E. coli is shown in figure 6b. There is restriction to the motion of Ag nanoparticles in gm positive bacteriums as these are protected by a thick peptidoglycan wall which provides suppression zone in E.coli than S.aureus. As there is addition in nanosilver atoms zone of suppression besides increases. Thereby, we can reason that antibacterial activity is due to presence of nanosilver in chitin scaffolds.

Cytotoxicity surveies:

Due to presence of Ag nanoparticles these scaffolds were found to be toxic. Though it is equivocal that this will consequence on lesion healing as old surveies have shown that lesion dressing holding nanoparticles are cytotoxic in vitro, while they give satisfactorily consequences in vivo.


Chitosan which is prepared from natural biopolymers was casted into membrane which was moreover tested for lesion dressing at the tegument transplant giver site in patients.

Bactigras which is normally used as impregnated tulle grass patch, acted as a control. Shrimp Chitosan which are holding 75 % grade of deacetylation and thickness of 10 Aµm was used in nonmesh and mesh signifier. After the measuring of thickness, wet content and swelling index, Chitosan membranes were sterilized and kept at room temperature in dry and clean topographic point until used.

For proving intents patients were admitted who needed split skin transplants. After the remotion of skin bed.i.e. 0.010-0.105 inch thickness Chitosan membranes were applied to cover the fresh lesion. One-half of the lesion was dressed with Chitosan membrane and other half with control Bactigras. It was random and about 20-25 pieces of gauze were used wrapping with elastic bondage. From twenty-four hours 0 to twenty-four hours 10 digital images were taken. After the remotion of dressing healed and unhealed countries were compared.

Clinical Observations:

Chitosan served as a successful lesion dressing stuff as it adhere uniformly to injure surface at that place by cut downing bacterial growing, hence cut downing hurting.

Non mesh membrane versus mesh membrane:

Blood coagulums were formed between the non mesh Chitosan membrane and renewing epithelial tissue of the lesion. Cleaning of coagulum was painful and there was harm of new epithelial tissue when coagulum was removed which leads to mark formation in the curative country. Whereas wounds which were dressed with Chitosan mesh membrane did non demo any blood curdling. Mesh membrane was easy to clean off and there was no hurting upon remotion ( Figures 3 & A ; 4 )

Figure 3 Wound site treated with Bactigras ( B ) and nonmesh Chitosan membrane ( C ) . ( a ) Dressing on Day 0, ( B ) covering gauze screening soaked blood at Day 1, ( degree Celsius ) blood coagulating at Day 1 and ( vitamin D ) cicatrix formation at 2 months.

Figure 4 Wound site treated with Bactigras ( B ) and Chitosan mesh membrane ( CM ) . ( a ) Meshed

Chitosan membrane, ( B ) covering gauze screening soaked blood at Day 1, ( degree Celsius ) healed lesion at Day 10, and ( vitamin D ) cicatrix formation at 1 month

Mending clip is defined as the clip which is needed for remotion of dressing without any demand of soaking and shed blooding. In instance of mesh Chitosan membrane mending rate was faster and better every bit compared to non mesh membranes.

Table1: Histological Findingss of Masson ‘s Trichrome Stain

Analyzing of lesion healing in human organic structure was followed by animate being theoretical account utilizing rats. Clinical information shows that mesh Chitosan membranes give more efficient attachment, haemostasis, mending and re-epitheliazation of lesion as compared to non mesh Chitosan membrane. In instance of mesh membrane itchiness and hurting is besides less and usage of no mesh membrane is non advised.


Synthesis of PEG

Hydrogels are big polymeric compounds which can retain immense sum of H2O within their construction without fade outing in them which gives them resemblance to populating tissue.

Use of I?- Chitosan as hydrogels:

I?- Chitosan has much higher responsiveness and versatility than I±- chitin because I?- chitin has really weak H bonding. There is a solubility issue in I?- chitin as formic acid is used to fix solution which irritates human tegument but this issue can be resolved by fixing hydrogels under milder conditions.

Synthesis of PEG/ I?- Chitosan:

PEG was dried by azeotrophic distillment with benzine and so acryloyl choride was added to it which yields propenoate terminated PEGM. This propenoate terminated PEGM was synthesized by UV- irridation in presence of aqueous acetic acid which gives PEGM/ I?- Chitosan semi- IPN. For elaborate synthesis refer conventional Figure 4

Figure5 Synthesis strategy of PEGM/ I?- Chitosan semi- IPN

Synthesis of PET

Poly ( ethylene terephthalate ) PET was treated with O plasma to bring forth peroxides on its surface. Further it was treated with Acrylic acid affecting transplant polymerisation to give PET-A.

PET-A-C and PET-Q-C were obtained utilizing Chitosan and quaternized Chitosan ( QC ) severally by intervention of PET-A with carboxyl groups.


Poly ( ethylene terephthalate ) PET, Chitosan, Quaternized Chitosan ( QC ) and acrylic acid

Favored Synthesis:

PET – A was obtained by handling PET with O plasma. It was so immersed in 10 % acrylic acid, and Na pyrosulfite was used as cut downing agent which gives ( PET-A ) which is shown in scheme1.

Scheme 1 Oxygen plasma intervention of PET and graft polymerisation of acrylic acid ( AA ) on PET.

This PET-A obtained was further used to obtain PET-A-C and PET-A-QC which is explained below:

Chitosan grafting to obtain ( PET-A-C )

First, PET-A was dipped in Chitosan solution for 8 H which leads to immobilisation of Chitosan on the surfaces and so it was oven dried for 5 min at 120oc. This leads to production of PET-A-C+ sample. PET-A was dipped in a 0.1 % ( w/v ) 1-ethyl-3- ( 3-dimethylaminopropyl ) carbodiimide ( wsc ) aqueous solution for 3hr at 4oc to trip the carboxyl groups on the surfaces and it was transferred into Chitosan solution which gives ( PET-A-C ) which is Chitosan grafted PET ( refer scheme2 )

Scheme 2 Conventional diagram demoing the formation of Chitosan-grafted poly ( ethene

Terephthalate ) ( PET-A-C ) .

QC grafting:

Quaternization of Chitosan was carried by adding a 5gm of Chitosan to 250 milliliters of N-methyl-2-pyrrolidinone followed by stirring at room temperature for 12 hour. Ice H2O was used to take down the temperature of suspension to 4oc. 43 milliliter of 1.4NNaOH aqueous solution, 6g of Na iodide, and 64g methyl iodide were added to this solution. Excess of propanone was removed utilizing filter paper. This process was repeated five times. For the activation of carboxyl groups PET-A was dipped into a Na citrate buffer solution incorporating 0.1 % H2O soluble carbodiimide. QC grafted PET which is ( PET-A-QC ) was obtained by dunking QC solution at 4oc for 24 hour to activated PET-A ( Scheme 3 )

Scheme 3 Conventional diagram demoing the Quaternization of Chitosan and their

Immobilization on PET-A

Strengths and failings:



Chitin and Chitosan are natural polymers due to this ; they have first-class belongingss such as biocompatibility, biodegradability, non-toxicity and surface assimilation.

As Chitosan is of course abundant stuff which exhibits restriction on its responsiveness and processability

Chemically modified chitin and Chitosan provides great addition in their solubility in general organic dissolvers.

In coastal countries it acts as major beginning of pollution

These are extremely basic polyoses, which provides them belongingss such as polyoxysalt formation, optical structural features and they can easy organize chelate metal ions and movies

It is extremely hydrophobic, is indissoluble in H2O and most organic dissolvers

When fibres of chitin and Chitosan are made these are really utile in field of absorbable sutura and lesion dressing stuffs

Due to hapless solubility, there is restriction on chitin use which makes it hard to look into its belongingss and construction.

When lesion dressing is made of chitin and Chitosan, these play critical function in remotion of heavy metal ions ( waste H2O intervention )

It is the lone natural cationic gum that becomes syrupy on being neutralized with acid.

Chitin Derived functions and Their Proposed Uses



Potential utilizations


Formyl, ethanoyl group, propionyl, butyryl, hexanoyl, Textiles, membranes, and

acetanoyl, decanoyl, dodecanoyl, tetradecanoyl, medical AIDSs

lauroyl, myristoyl, palmitoyl, stearoyl, benzoyl,

monochloroacetoyl, dichloroacetyl,

trifluoroacetyl, carbamoyl, succinyl

Fabrics, membranes, and

, medical AIDSs

N-Carboxyalkyl ( aryl ) chitosans

N-Carboxybenzyl, glycine-glucan, N-carboxy- methyl Chitosan, alanine glucan, phenylalanine glucan, tyrosine glucan, serine glucan,

glutamicacid glucan, methionine glucan,

leucine glucan

Chromatographic media and metal ion aggregation

o-Carboxyalkyl chitosans

o-Carboxymethyl, cross-linked o-Carboxymethyl

Molecular screens, viscousness

builders, and metal ion


Metal ion chelates

Palladium, Cu, Ag, I

Catalyst, picture taking, wellness

merchandises, and insect powders

Application of Chitosan

Chitosan and its derived functions have a broad scope of applications. Hydroxyl methyl chitin and H2O soluble Chitosan derived functions can be used to work out environmental and biomedical jobs, such as anionic waste watercourses. For controlled drug release applications Chitosan derived functions can be employed as a bearer. It can be utile to be bio-scaffold for tissue technology peculiarly in instance of tegument and castanetss. Chitosan possesses all the coveted characteristics for an ideal contact lens like optical lucidity, optical lucidity, optical rectification, mechanical stableness, gas permeableness, wettability and immunological compatibility.

Future waies for farther research and development

In instance of in vivo toxicity surveies comparison with experimental animate beings is really utile for achieving in vitro biocompatibility theoretical accounts. As biocompatibility of biomedical class Chitosan is boring phenomenon compromising other immune reactions with assorted inflammatory cells, therefore we can non wholly replace toxicity surveies in experimental animate beings. By comparing the biocompatibility consequences both from both in vitro and in vivo we can be small secured but for full confidence we need to seek clinical tests of freshly developed synthesized biomedical class Chitosan on the worlds.For the survey of individual cell proliferation and invasion form within a porous structured biomedical class Chitosan gives better consequences as compared to in vivo theoretical account.

There are recent promotions in apprehension of biological science, chemical science and related Fieldss which will give more development of nowadays in vitro biocompatibility theoretical account. To minimise hazard to worlds and animate beings we should concentrate elaborate rating of non merely cellular but besides molecular responses that we can make in vivo trials.

Cite this Second Most Abundant Polysaccharide In Nature Biology

Second Most Abundant Polysaccharide In Nature Biology. (2017, Jul 14). Retrieved from https://graduateway.com/second-most-abundant-polysaccharide-in-nature-biology-essay/

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