VINEPAN [Vinegar and Pandan (Pandanus amaryllifolius)] as Repellent Floor Wax

VINEPAN [Vinegar and Pandan (Pandanus amaryllifolius)] as Repellent Floor Wax

An entry to the
DIVISION SCIENCE FESTIVAL 2013

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John Louise Rogador Marfil
Eugem Manalon Gutierrez
Gerick Machado Billanes
RESEARCHERS

MRS. JUVELYN FETIL MALLORCA
ADVISER

TABLE OF CONTENTS

I. Title Page . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1 II. Acknowledgment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 III. Abstract . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 Research Plan. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-6 Question or Problem being addressed. . . . . . . . . . . . . . . . . . . . . . . .9 Goals/ Expected outcomes/ Hypothesis

Description in detail of method or procedure
Procedure
Data Analysis
Bibliography
IV. Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-8 Background of the Study
Statement of the Problem
General Objective
Specific Objective
Significance of the Study
Scope and Limitations
V. Results and Discussion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11-15 Findings
Statistical Data
Discussion of Results
VI. Conclusions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .16 VII. Recommendations. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16 VIII. Bibliography . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .18

ACKNOWLEDGEMENT

First, we would like to extend our warmest gratitude to our classmates who supported us all the way out.
To our dearest adviser, Ma’am Juvelyn Fetil Mallorca, whom we seek pieces of advice on how to perform this research.
To our beloved parents, Mr. Rodel and Mariquita Billanes, Mr. Lazaro and Luzmelinda Gutierrez, Mr. Adriano and Alona Marfil who supported us financially.
To the pandan leaves contributors namely Niñalyn Leynes, Miggy Riano, Krisanta Salac, Erchel Lagrosa, Argielyn Tome and of course to Mr. and Mrs. Joey Fabul who at the same time provided us shelter during rainy days.

To our teachers, Ms. Annaliza Capillo and Mrs. Faminiano, who checked our grammar and computation even if they have their classes.
To Mr. Elwood Fruelda, who gave us companionship when we were working on our write-up.
Most especially, we would like to thank God for without him, we cannot finish this project.

ABSTRACT

Cockroaches are common problem encountered by every household. These
cockroaches may mess all things around. This study was conducted to test the effectiveness of VINEPAN cockroach repellent floor wax in eliminating cockroaches. It was also performed to help each household in finding cheap, safe and affordable one as to cockroach repellent.

Vinegar, pandan, cooking oil, candle wax were the ingredients used in making VINEPAN cockroach repellent floor wax. Three treatments were prepared varying the amount of vinegar and pandan leaves extract, and to be applied to three set-ups respectively. Each set-up was divided into two equal sides: one side with floor wax and one side left untouched. The effectiveness of VINEPAN (Vinegar and Pandan) cockroach repellent floor wax was statistically tested using the Analysis of Variance (ANOVA) or F-test. The data were gathered according to the number of cockroaches repelled using the three treatments. Interpretation of results indicates that VINEPAN (Vinegar and Pandan) cockroach repellent floor wax was effective in repelling cockroaches. Also it showed that it is more effective when greater amount of vinegar and pandan is used.

RESEARCH PLAN

A. Question or Problem being addressed
This research aims to answer the following questions:
Is the floor wax made from vinegar and pandan leaves extract an effective cockroach repellent when applied? Does varying the amount of vinegar and pandan leaves extract significantly affect the effectiveness of the floor wax?

B. Goals / Expected Outcomes / Hypothesis
This study intends to make a floor wax that could be an answer to the problem about cockroaches. This research also intends to discover various products out of materials around us.
It is expected that this research would be a great help since it is effective. It is also expected that varying the amount of vinegar and pandan leaves extract will have a significant effect on the effectiveness of the floor wax.

Null Hypothesis
Varying the amount of vinegar and pandan leaves extract has no significant effect in the effectiveness of the floor wax when it comes to the number of cockroaches eradicated.

C. Methods or Procedures
Procedure:
Considerable amount of pandan leaves are to be gathered first. Then it will be washed thoroughly with water. After the pandan leaves have been washed, it will be extracted using a mortar and pestle and a piece of cloth. Next, the set-up is to be prepared. Including this set-up are the following: for the first set-up, 20 mL vinegar, 60 mL pandan leaves extract, 30 mL oil and 100 g candle wax; for the second set-up, 25 mL vinegar, 70 mL pandan leaves extract, 30 mL oil and 100 g candle wax; for the third set-up, 30 mL vinegar, 80 mL pandan leaves extract, 30 mL oil and 100 g candle wax (the kind of vinegar to be used is fermented TUBA and the kind of oil to be used is cooking oil). After it has been prepared, proceed to the floor wax making. Only one procedure is to be done in making it. First, candle wax will be heated in a pan and wait until it has melted. After the candle wax has melted, the vinegar will be added and mixed gradually. Then, pandan leaves extract is to be added. Stir until it is thoroughly mixed. Wait until the mixture hardens. Afterwards, the hardened mixture is to be pounded using mortar and pestle. While pounding, considerable amount of oil is to be added until the desired consistency is reached; finally forming the floor wax.

Data Analysis:
Three set-ups were made using the three formulations of floor wax. Each set-up was divided into two equal sides. Floor wax was applied on one side and the other side left untouched. Then cockroaches were put inside the set-up. The time was constant which is 5 minutes. To get the needed data, after sometime, the researchers count the number of cockroaches which were on the side of the set-up applied with floor wax and on the side which was left untouched. This process was repeated 5 times to get a reliable data, using the three formulations of floor wax.

Bibliography
Collier’s Encyclopedia 6 pp. 676-677
Collier’s Encyclopedia 9 pp. 663
Collier’s Encyclopedia 20 pp.532
New Age Encyclopedia pp. 111
Grolier International Encyclopedia (Deluxe Home Edition) pp.157

INTRODUCTION

Background of the Study
As children, it’s our responsibility to help our parents in doing the household chores. Cleaning and maintaining the floor shiny is one. However we cannot ignore the number of cockroaches roaming and pestering all of the things around. Regarding this matter, several products are manufactured such as different brands of cleaners and floor waxes. However these products are relatively costly.

Way back sometime, when the researcher’s cousin was still working as “All around Girl” at someone’s house, she had a hard time cleaning and polishing the floor. In addition to that, there were cockroaches almost everywhere. Having realized the problem, the researcher did some readings and found out that vinegar could be a good cleaning agent and pandan (Pandanus amaryllifolius.) has become one of the ingredients of some pesticides now-a-days.

So the researchers decided to make a floor wax out of the said materials that does not only keep the floor clean and shiny but also drive the cockroaches away.

Statement of the Problem
General objective
This research was conducted to test if the floor wax made from vinegar and pandan (Pandanus amaryllifolius) could make and keep the floor clean and shiny and could drive the cockroaches away for a considerable time.

Specific Objective
This research was conducted to test if varying the amounts of vinegar and pandan (Pandanus amaryllifolius) extract could affect the effectiveness of the floor wax.

Significance of the Study
This research was conducted to help children like us, who often do the household chores particularly cleaning the floor. This research is intended to make and keep the floor clean, shiny and of course, cockroach free. Since the raw materials to be used in this research are common in our locality, this floor wax would be cheaper than commercial ones. Moreover, it would also be eco-friendly since there will be no harmful chemicals involved in conducting this research, thus, it would also be safe for our health.

Scope and Limitations
This research is intended for testing any kind of floor. It is also limited to the use of vinegar and pandan (Pandanus amaryllifolius) extract as the raw materials in making the said floor wax. The use of other materials is not allowed.

This research also focuses to the variation of the amount of vinegar and pandan (Pandanus amaryllifolius) extract in relation to the floor wax’s effectiveness.

REVIEW OF RELATED LITERATURE

Cockroach or roach is one of the oldest and most primitive of insects. It is one of the insects which are most disagreeable to man because of destructiveness and field odor. It flourished during the carboniferous period and many species are still found throughout the world.

Cockroaches range in length from about half an inch to 2 inches (5cm) in some tropical species. The body is oval and flattened, and in most species, there are wings held flat over the back. They have chewing mouth parts and
feed on any plant or animal matter and manufactured products. As nocturnal insects, they hide during the day in warm, damp, dark places, coming out at night in search of food. They live under rocks and plant debris outdoors. In houses, they are most often found near the water pipes, in the walls, and under the floors of kitchens and bathrooms. The majority deposit egg sacs, but some give birth to living young.

Paraffin wax or candle wax is commonly white or colorless soft solid that is used as a lubricant and for other applications. It is derived from petroleum and consists of a mixture of hydrocarbon molecules containing between twenty and forty carbon atoms. It is solid at room temperature and begins to melt above approximately 37 °C (99 °F);] its boiling point is >370 deg C. In chemistry, paraffin is used synonymously with “alkane”, indicating hydrocarbons with the general formula CnH2n+2. The name is derived from Latin parum (“barely”) + affinis, meaning “lacking affinity” or “lacking reactivity” indicating paraffin’s unreactive nature.

Pandan (Screw pine – English name) a woody plant, genus Pandanus, in the screw pine family, native to the Old World tropics. The name is derived from the spiral arrangement of the leaves, the scars of which sometimes leave a continuous corkscrew-like spiral on the trunk and branches. Pandan leaves contain a number of essential oils and chemicals that cockroaches find unpleasant. Terpenes and sesquiterpene hydrocarbons make up between 6 and 42 percent of the oils in these leaves, according to the National University of Singapore researchers. Pandan also contains 2-acetyl-1-pyrroline, or 2AP, a substance that repels both American and German cockroach species in test studies.

Vinegar (from Fr. Vinaigre, “sour wine”), a sour liquid, containing approximately 5% acetic acid, made from the fermentation of ale, beer, cider, malt, or wine or from fermented fruit juices. This common kitchen item is used variously as a condiment, meat tenderizer, and preservative and as an ingredient in sauces, salad dressings, and other food preparations. It may also be employed as a house-cleaning agent.

RESULTS AND DISCUSSION

A. FINDINGS
Figure 1.1
GRAPHICAL RESULT

Legend:
Treatment A – 20 mL vinegar, 60 mL pandan leaves extract, 30 mL oil and 100 g candle wax Treatment B – 25 mL vinegar, 70 mL pandan leaves extract, 30 mL oil and 100 g candle wax Treatment C – 30 mL vinegar, 80 mL pandan leaves extract, 30 mL oil and 100 g candle wax

RUBRICS FOR RATING

5 = There were no cockroaches on the side where floor wax was applied. All cockroaches were repelled. 4 = There were 7-8 cockroaches repelled. 2-3 cockroaches were on the side where floor wax was applied. 3 =There were 5-6 cockroaches repelled. 4-5 cockroaches were on the side where floor wax was applied. 2 = There were 3-4 cockroaches repelled. 6-7 cockroaches were on the side where floor wax was applied. 1 = There was 0-2 cockroaches repelled. 8-10 cockroaches were on the side where floor wax was applied.

B. STATISTICAL DATA
Trial
Treatment
A
Treatment
B
Treatment
C
Treatment
A2
Treatment
B2
Treatment
C2
1
3
3
5
9
9
25
2
2
4
4
4
16
16
3
1
3
5
1
9
25
4
3
2
5
9
4
25
5
2
3
3
4
9
9
Total
11
15
22
27
47
100
GRAND TOTAL
48
x
2.2
3
4.4

TOTAL SS = (sum of squares of all x values) 2 – CM
TOTAL SS = (27 + 47 + 100) – 153.6
TOTAL SS = 174 – 153.6
TOTAL SS = 20.4

– CM

SSE = TOTAL SS – SST
SSE = 20.4 – 12.4
SSE = 8

MST = SST/ k-1
MST = 12.4/ 3-1
MST = 12.4/2
MST= 6.2

MSE = SSE/ n-k
MSE = 8/ 15 – 3
MSE = 8/ 12
MSE = 0.667

F = MST/ MSE
F = 6.2/ 0.667
F = 9.295
Where, the d.f.1 = k – 1 = 3 – 1 = 2
d.f.2 = n – k = 15 -3 = 12

Fcritical for a = 0.05 is 3.8853. Since F = 9.295 > Fcritical = 3.8853, reject the Ho. Source of Variation
d.f.
SS
MS
F
Treatments
2
12.4
6.2
9.295
Error
12
8
0.667

TOTAL
14
20.4

C. DISCUSSION OF RESULTS
The data presented above are used to test whether the null hypotheses are rejected or accepted. This data were gathered from the ratings given by the researchers after using the VINEPAN Cockroach Repellent Floor wax using the three treatments.

Thus the statistical treatment used was the Analysis of Variance (ANOVA) or F-test to know if VINEPAN can be used as cockroach repellent floor wax. As calculated, Fcalculated is greater than Fcritical at 5% level of significance; therefore, the researchers rejected the null hypotheses.

Based on the statistical data it was shown that VINEPAN (Vinegar and Pandan) can be used as cockroach repellent floor wax and varying the amount of vinegar and pandan leaves extract affect the effectiveness of VINEPAN. The greater the amount of vinegar and pandan leaves extract, the more effective the cockroach repellent floor wax is. Pandan contains 2-acetyl-1-pyrroline, or 2AP, a substance that repelled cockroaches.

SUMMARY, CONCLUSION AND RECOMMENDATION

Summary
According to the results, null hypotheses must be rejected. Hence the results of the study undertaken were: 1. Floor wax made from vinegar and pandan (Pandanus amaryllifolius) could drive cockroaches away. 2. Varying the amount of vinegar and pandan (Pandanus amaryllifolius) extract could affect the effectiveness of the floor wax. Conclusion

The result Fcalculated is greater than Fcritical led the researchers to a conclusion that the null hypotheses must be rejected. Thus, VINEPAN (Vinegar and Pandan) can be a cockroach repellent floor wax and varying the amount of vinegar and pandan leaves extract does affect the effectiveness of VINEPAN cockroach repellent floor wax. The greater the amount of vinegar and pandan leaves extract, the more effective the cockroach repellent floor wax is. Recommendations

After the research was done, the researchers realized that the pandan leaves should be extracted carefully. In measuring the materials needed, cooking oil, candle wax must be properly quantitated because if not, the VINEPAN will become hard.

The researchers recommend improving the product by adding chemicals that could help VINEPAN (Vinegar and Pandan) cockroach repellent floor wax have the right consistency in relation to floor wax’s effectiveness.

When the product is improved, that is the only time that the VINEPAN (Vinegar and Pandan) cockroach repellent floor wax could be compared to the commercial ones. This product can also be sold commercially if shown that it can compete with the commercially sold floor waxes.

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