All organisms, including plants and animals, oxidize glucose for energy. Often, this energy is used to convert ADAPT and phosphate into TAP. It is known that meal worms undergo cell respiration during germination. Do meal worms undergo cell respiration before germination?
Using your collected data, you will be able to answer this question concerning respiration and non-germinated meal worms. Using the 02 Gas Sensor, you will monitor the oxygen consumed by meal worms during cell respiration. Both larval and pupated meal worms will be tested. Additionally, cell respiration of larval meal worms at two different temperatures will be tested. OBJECTIVES In this experiment, you will Use an 02 Gas Sensor to measure concentrations of oxygen gas during cell respiration. Study the effect of temperature on cell respiration rate. Determine whether meal worms and pupated meal worms respire.
Compare the rates of cell respiration in germinating and non-germinating meal worms. Figure 1 Connect the 02 Gas Sensor to Labiates and choose New from the File menu. If you have an older sensor that does not auto-ID, manually set up the sensor. 2. On the Meter screen, tap Rate. Change the data-collection rate to 0. Samples/second and the data-collection length to 600 seconds. . Measure the room temperature using a thermometer and record the temperature in Table 1. 4. Obtain 10 meal worm larvae. 5. Place the meal worm larvae into the respiration chamber. 6. Place the 02 Gas Sensor into the bottle as shown in Figure 1. Gently push the sensor down into the bottle until it fits snugly. 7. Wait two minutes, then start data collection. 8. When data collection has finished, a graph of 02 gas vs.. Time will be displayed. 9. Remove the 02 Gas Sensor from the respiration chamber. Place the worms in a 00 ml beaker and place on an ice bath. 10.
Fill the respiration chamber with water and then empty it. Thoroughly dry the inside of the respiration chamber with a paper towel. 1 1 . Perform a linear regression to calculate the rate of respiration. A. Choose Curve Fit from the Analyze menu. B. Select Linear for the Fit Equation. The linear-regression statistics for these two data columns are displayed for the equation in the form y = mix + b where x is time, y is percent oxygen, m is the slope, and b is the y-intercept. C. Enter the absolute value of the slope, m, as the reaction rate in Table 2. . Select K. 2. Store the data from the first run by tapping the File Cabinet icon. 13. Repeat Steps 6-12 substituting the meal worms with meal worm pupae. In Step 9 place the meal worms back in their container and not in the ice bath. Part II Meal Worms, cool temperatures 14. Remove the meal worms from the cold water and gently blot them dry between two paper towels. Repeat Steps 6-11 using the meal worms. Important: Do not store the data 15. As you did for the last two runs. 16. Graph all three runs of data on a single graph. To do this: a. Tap Run 3 and select All Runs. B.
All three runs will now be displayed on the same graph axes. C. Use the displayed graph and Tables 1 and 2 to answer the questions below. DATA Table 1 Condition Temperature (co) room Table 2 Meal Worms Rate of respiration (%/s) Worms , room temperature Pupae, room temperature Worms, cool temperature Questions 1. Do you have evidence that cell respiration occurred in meal worms? Explain. 2. What is the effect of pupation on the rate of cell respiration in meal worms? 3. What is the effect of temperature on the rate of cell respiration in meal worms? 4. Why do germinated meal worms undergo cell respiration?
