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Chromatography

Separation of Chlorophyll a, Chlorophyll B, and Beta Carotene by Paper Chromatography

Abstract Pigments extracted from different greens have different polarities and may be different colors. Mixed pigments can be separated using chromatography paper. Chromatography paper is able to separate mixed pigments due to their polarity and solubility. Pigments of chlorophyll a, chlorophyll b and beta carotene will be separated on chromatography paper because each has its …

Chromatography Lab Pre- Lab Questions

The chromatography lab is to understand how molecules with similar molecular properties can be separated with paper chromatography. These differences will be interpreted to see the distinction of separate chemical substances. Pre Lab Questions 1. Explain capillary action as it pertains to water and paper. Capillary action makes water draw up the paper. As paper …

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Separating Ferrocene and Acetylferrocene by Adsorption Chromatography

Separating Ferrocene and Acetylferrocene by Adsorption Chromatography Dry Pack Method Leah Monroe February 27, 2003 Organic Chemistry Lab II Experiment performed on February 18 and 20, 2003 Abstract: Adsorption column chromatography is one way to separate compounds out of a mixture. In this technique, a solid stationary phase called the adsorbent is packed in a …

Thin Layer Chromatography of Analgesics

Thin-Layer Chromatography of Analgesics Abstract: In this experiment thin-layer chromatography (TLC) was used to determine the composition of an unknown mixture of analgesics given five references of AC, ASP, CAF, IBU, SAL, an unknown consisting of a mixture of three of the references, and two unknowns (6,23) containing a single reference compound. The composition of …

Ink Paper Chromatography

Ink Chromatography Lab Background Information Paper chromatography is an analytical method that is used for separating and identifying mixtures of substances into their smaller parts. Paper chromatography works because the ink used contains several dyes (pigments) that when place on porous paper are dissolved in a solvent by capillary action. When the pigments (solutes) are …

Paper Chromatography

Abstract Paper Chromatography uses a chromatogram paper as its stationary phase and the solvent as the mobile phase. Retention factor is the ratio of the distance travelled by the sample to the distance travelled by the solvent. This experiment aims to separate organic compounds, to compute Rf values and to identify unknown compounds using Rf …

5.05 Ink Chromatography

Background Information and Research: 1. Paper chromatography type of method that is used to separate mixtures of substances from a solution. 2. There are many uses for paper chromatography, especially concerning the fields of chemistry and biology. One use is to allow scientists to detect any sort of contaminants is rainwater. Another use is to …

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