Does the aromatic substance from garlic have suppressing consequence on the sprouting and growing of the boodle seeds? This is the inquiry that was put as the base of our research lab experiment.
Introduction
Many workss produce chemical compounds that are non straight involved in the normal reproduction, growing or development of beings. These compounds are called secondary metabolites. Plants may utilize secondary metabolites to vie with each other by let go ofing these chemicals into the environment. Allelopathy is “ production of biomolecules by one works, largely secondary metabolites, that can bring on enduring in or give benefit to, another works. The phenomenon can besides be considered as a biochemical interaction among workss ” ( Rizvi and Rizvi ) . The influence of the workss upon each other can be either good – positive allelopathy or damaging – negative allelopathy. Negative allelopathy is a signifier of chemical competition. Allelopathic workss prevent other workss from utilizing the available resources such as foods, H2O and sunshine. Positive allelopathy encourages other workss to turn. It can be done by seting off insects and other marauders and by suppressing bacteriums, casts and other harmful substances.
Garlic ( Allium sativum ) is an allelopathic works. Intact garlic contains odourless compound alliin and an enzyme, alliin lyase. Enzyme and substrate become assorted when harm is done to the garlic cells and as a consequence, volatile secondary metabolite called allicin is produced. Allicin is produced in Allium sativum merely when the works is injured and its tissues are damaged or crushed. The typical olfactory property of the crushed Allium sativum is due to allicin production. Allicin inhibits the growing of micro-organisms and seed sprouting and protects the damaged Allium sativum from micro-organisms and competition from other workss for foods ( Miron, Shin, Feigenblat, Weiner, Mirelman, Wilchek and Rabinkov ) .
The hypothesis that is stated for the experiment is:
Yes, the aromatic substance ( volatile ) from Allium sativum will suppress the sprouting and growing of the boodle seeds.
Methods and Materials
In the experiment:
- Independent variable: aromatic substance ( volatile ) of Allium sativum
- Dependent variable: figure of germinated seeds, length of seed ‘s roots
- Nuisance variables: seed ‘s genetic sciences, room temperature, visible radiation
- Nuisance variables were controlled by keeping the same location for all Petri dishes and by maintaining their unity.
- Experimental seeds: Lettuce ( Lactuca sativa ) seeds
- Garlic ( Allium sativum )
- Seed Germination set up: 9 centimeter Petri dishes with two pieces of Whatman filter paper
- Distilled H2O: 3ml per set up
- Dissembling tape
- Aluminum foil
- Knife or razor
- Triple beam balance
Garlic was crushed utilizing a razor. 1.0 g samples were weighed on a ternary beam balance and transferred to two aluminium boats. Then, two aluminium boats with crushed Allium sativum were placed into two experimental Petri dishes that contained 2 pieces of Whatman # 1 filter paper and 20 boodle seeds in each. 3 milliliter of distilled H2O were added and dishes were sealed with dissembling tape. Two other Petri dishes were set up for control groups. Controls set up were done as described above with the exclusion of adding of Allium sativum to the aluminium dishes. All four Petri dishes were incubated and observed for two hebdomads under schoolroom conditions. At the terminal of the experiment, the consequences of the control intervention will be compared to the consequences of the experimental interventions. Two experimental and two control groups were used. 4 Petri dishes with two pieces of Whatman filter paper 4 aluminium boats 80 boodle seeds, 20 in each Petri dish 2 g Allium sativum, 1g in each experimental group Petri dish 12 milliliter distill H2O, 3 milliliter per Petri dish
Consequences
There were seven measurings taken over the span of two hebdomads. It was observed that the two dishes with garlic had less germinated seeds and grew much less than the two dishes without Allium sativum. Therefore, the consequence of the experiment is that Allium sativum has a negative allelopathic consequence on boodle seeds sprouting and growing.
On the first twenty-four hours when the measurings were taken it was observed that most of the boodle seeds in the two control groups were germinated but merely one and two seeds were germinated in the experimental 1 and experimental 2 groups severally.
On the twenty-four hours two, all 20 seeds were germinated in both control groups 1 and 2 but merely 16 and 13 seeds were germinated in the experimental 1 and experimental 2 groups severally. By the terminal of the experiment one more seed germinated in the experimental group 1 which brought the entire figure of germinated seeds in the experimental group 1 to seventeen seeds. In the control group1, control group 2 and experimental group 2 figure of germinated seeds have n’t changed from the twenty-four hours three except two roots decayed in the control group1 on the last twenty-four hours of the experiment.
As for the length of the roots, it was observed that stems started to turn perceptibly on the twenty-four hours three of the experiment. Average length of the roots in both control group 1 and 2 was obviously longer than the mean length of the roots in the experimental groups 1 and 2. The root in both control groups continued to turn till the terminal of the experiment. The root in both experimental groups grew really small and so all decayed by the twenty-four hours six of the experiment. On the contrary, by the terminal of the experiment merely two roots decayed in the Control group 1. The consequences above demonstrate that Allium sativum has a negative consequence on sprouting and growing of boodle seeds. Refer to the Table 1 for the sum-up of experimental informations.
Discussion
At the beginning of the experiment it was stated that Allium sativum would hold a negative allelopathic consequence on the sprouting and growing of other seeds. After carry oning the experiment and assemblage informations it can be concluded that this experiment supports the hypothesis. The seeds in both control groups with no Allium sativum had more germinated seeds and much longer stems than the seeds in experimental groups with garlic in them.
The shortest root in the containers with garlic ( experimental groups ) was 4mm and the longest 1 was merely 9 millimeters, comparing to the shortest 23 millimeter and the longest 53 millimeter in containers without garlic ( command groups ) .
The seeds in the containers with garlic ( experimental groups ) did non turn longer than 9 millimeters and all decayed by the twenty-four hours six of the experiment, compared to the seeds in the containers without garlic ( command groups ) , which grew up to a upper limit of 53 millimeters and appeared alive and healthy except two seeds that decayed on the last twenty-four hours of the experiment.
I was able to retroflex the experiment by utilizing 20 seeds in each Petri dish ( the sample size of 20 seeds ) along with two controls and two experimental groups. Such a sample size was sufficient to pull a decision from one experiment. However, the clip I had to make the experiment in the research lab was limited and hence, I was non able to reiterate the experiment multiple times in order for it to be wholly dependable. This was one of the failings in my probe. Another failing was that many people had entree to the lab, where the experiment was conducted, and as a consequence, the samples could hold been moved from their original topographic points. It could hold affected the consequence of the experiment every bit good. If more clip given, this experiment should hold been repeated multiple times and in more secure environment if possible.
In decision, the consequences of this research are in line with the consequences of the same experiments conducted before. This experiment followed my original hypothesis that aromatic substance ( volatile ) from Allium sativum will suppress the sprouting and growing of boodle seeds.
Such of import feature of Allium sativum can be utilized in the agribusiness. As our society started to acknowledge importance of organic green goods and injuriousness of commercial pesticides, Allium sativum can be used as an organic pesticide. Of class, more research and experiments are needed in this country.
