We use cookies to give you the best experience possible. By continuing we’ll assume you’re on board with our cookie policy

See Pricing

What's Your Topic?

Hire a Professional Writer Now

The input space is limited by 250 symbols

What's Your Deadline?

Choose 3 Hours or More.
Back
2/4 steps

How Many Pages?

Back
3/4 steps

Sign Up and See Pricing

"You must agree to out terms of services and privacy policy"
Back
Get Offer

Microbiology Unknown and Purpose of Microbiology

Hire a Professional Writer Now

The input space is limited by 250 symbols

Deadline:2 days left
"You must agree to out terms of services and privacy policy"
Write my paper

Microbiology Unknown #94 The purpose of this report was to identify an unknown bacterium by applying methods that were previously learned and conducted in microbiology lab class. It is possible to identify a mixed culture by running experiments on the unknown bacteria and taking what we already know and applying it to the results. It helps to slowly eliminate any bacteria that do not correspond with the results of certain tests.

An known test tube labeled 94 was given to me b my instructor.

Don't use plagiarized sources. Get Your Custom Essay on
Microbiology Unknown and Purpose of Microbiology
Just from $13,9/Page
Get custom paper

The first procedure performed was an isolation of my unknown bacteria with the goal of obtaining a pure culture. This was done by streaking the unknown onto a nutrient agar plate using the streak method. The plates were incubated for two days and the bacterium was able to grow. I studied the bacteria based of its physical characteristics of how it grew on the agar. I began my quest by conducting a Gram stain on my bacteria.

I prepared a near by placing a drop of water onto the center of a slide and then removed a loop full of bacteria to place within the water droplet.

I use the aseptic treatment while doing this making sure to flame the inoculating loop before and after transferring bacteria. It was important to get this done correctly because the test determines the stain as well as the shape of the cells. After looking at my stain under the microscopic I came to the conclusion that my bacteria was a positive Cisco.

After the gram stain I reformed specific tests based of those results of the stain to determine what my unknown could be. The first test I did was the catalane test. It had a positive reaction meaning the bacteria produced the enzyme catalane. This helped me narrow my unknown down to Microcosmic and Staphylococcus. I inoculated my unknown onto a imitation salt agar. The growth and color change to yellow indicated imitation fermentation because acid was produced. The acid fermentation is a characteristic of the pathogen Staphylococci.

Then a nitrate broth test was performed. This test also had a positive reaction meaning the reduction to nitrates occurred. From these results I was able to indicate that my unknown was Staphylococcus. To make sure of my decision I performed a triple sugar iron agar test. The results show that glucose, lactose, and sucrose fermentation with acid accumulated in the test tube. With the results of all the tests I performed, I came to the conclusion that my unknown was Staphylococcus erasures. By assayer

Cite this Microbiology Unknown and Purpose of Microbiology

Microbiology Unknown and Purpose of Microbiology. (2017, Oct 27). Retrieved from https://graduateway.com/microbiology-unknown-3/

Show less
  • Use multiple resourses when assembling your essay
  • Get help form professional writers when not sure you can do it yourself
  • Use Plagiarism Checker to double check your essay
  • Do not copy and paste free to download essays
Get plagiarism free essay

Search for essay samples now

Haven't found the Essay You Want?

Get my paper now

For Only $13.90/page