One of the other purposes of the experiment was on how to fix a blood vilification. Knowing how to fix a blood vilification would be good as scientist could analyze more about ruddy and white blood cells closely. One illustration of industrial applications would be staining the blood, as it could look into whether patients are enduring from leukaemia, as higher sum of white blood cells would be present in patients enduring from leukaemia. Another illustration of application of designation of nucleic acid would be the mass production of insulin for patients who could non bring forth insulin, as scientist would hold to place a little codification of nucleic acids which is called the cistron of insulin. Therefore, after placing, they would abstract it out and attached it to Escheria.coli for reproduction of insulin cistrons.
White blood cells, besides known as leukocytes, are made in the bone marrow. They help the organic structure by protecting it against viruses, bacteriums and diseases. They could besides be called the “ white ground forcess of the organic structure ” . There are two major groups of white blood cells which are granulocytes and agranulocytes.
One difference between granulocytes and agranulocytes are their karyon. Granulocytes have “ farinaceous ” karyon while agranulocytes have big “ whole karyon ” . Granulocytes consist of neutrophil, basophil, and eosinophil. Each of them has their ain maps in assisting the immune system. Agranulocytes consists of lymph cell and monocyte. They excessively, have their ain maps in assisting the organic structure.
In every karyon of a eukaryote cell, big and condensed molecules of base will be present. They are known as nucleic acids, they besides carry familial information of the living thing. However, there are two types of nucleic acids, one known as deoxyribonucleic acid ( DNA ) while the following is ribonucleic acid ( RNA ) .
The construction of a Deoxyribonucleic acid is dual spiral while RNA is single-stranded. Deoxyribonucleic acid could be found in the karyon merely, nevertheless, RNA could be found in both nucleus and cytol. Both are different in their maps, DNA helps in cell division while RNA helps in protein synthesis.
Methyl green pyronin is a discoloration that would assist visualise white blood cells easier, leting scientist to distinguish between granulocytes and agranulocytes. Methyl viridity has a discriminatory affinity for DNA, which would stain DNA green, and pyronin for RNA, which stains it rose-red. The higher the strength of the coloring material besides represents higher concentration degree of DNA and RNA.
RNase is an enzyme that would degrade RNA. A cell added with RNase would merely incorporate DNA as all RNA has already been degraded. One maps of RNase is that it is used to clean up cellular RNA.
The hypothesis of the experiment was that the RNase treated slide will merely hold green coloured karyon after adding methyl green pyronin ( MGP ) . The slide that is non treated with RNase will hold green and ruddy coloring material.
The consequences obtained from the experiment was that, slide treated with RNase showed merely green karyon in the white blood cells after stained by methyl-green pyronin. The slide which merely was stained by methyl-green pyronin showed two colorss in the karyon which are ruddy and green with an add-on of ruddy cytol.
The consequences besides showed the visual aspect of the white blood cells. Both granulocytes and agranulocytes were observed in the blood smear slides.
The consequences obtained were the same as the hypothesis. However, the sum of white blood cells present in the blood vilification was lower compared to other group ‘s consequences. One of the grounds might be because due to the clip taken for rinse of slides. No proper halt ticker was used and clip could be merely estimated. Therefore, the clip taken was non accurate.
Furthermore, artefacts were observed during the microscopic scrutiny. This could misdirect into other type of consequences, as some type of artefacts might look like a cell. Therefore, in order to hold no artefacts at all, the glass slides have to be clean so that no dust atom would look on the blood. Air bubbles have to be kept out when seting cover faux pas on glass slides.
One measure in methods and stuffs was supposed to be done, which was adding xylol as it could do the cells clearer to be seen under the microscope. However, this measure was non done. The ground is because xylol is carcinogenic and might be harmful towards the human organic structure. Therefore, to avoid any hazards, xylene was non used in the experiment.
Some stairss could be carried out during the experiment so that the consequences could be improved. As hair drier was used during the experiment, this was non encouraged because the heat might damage the cells. Therefore, consequences would be inaccurate.
One other measure could be that the blood smear slides were put in an increasing intoxicant concentration. This would let maximise sum of H2O to go forth the cell, doing the cell to its lowest H2O possible degree.
Last, bright-field microscopy is best suited to sing stained or of course pigmented specimens such as stained prepared slides of tissue subdivisions or populating photosynthetic beings. It is useless for populating specimens of bacteriums, and inferior for non-photosynthetic protists or metazoans, or unstained cell suspensions or tissue subdivisions ( Caprette, 2005 ) .
In decision, the purposes were achieved as accomplishments such as fixing blood vilifications and detecting DNA and RNA were done. The consequences were proven as it has the same readings of the hypothesis. Therefore, the experiment was a success.
