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Natural Antiseptics Vs Medical Antiseptics Biology

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Abstraction

This extended experimental probe was commenced to see how natural antiseptics compared to man-made medical antiseptics, on Serratia marcescens bacteria civilization. The consequences showed that the natural merchandise Manuka honey UMF 30+ worked better so the semisynthetic antiseptic pick.

Introduction

Bacterias

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Bacterias are individual celled micro-organisms, that do n’t hold a membrane-bound karyon and other cell organs such as chondriosomes and chloroplasts. Bacteria cells have many belongingss. They consist chiefly of ; ribosomes, scourge, cell wall made of peptidoglycan, cytol, cytoplasmatic membrane, and nucleoid.

Bacteria can either populate independently or dependently necessitating another life being to maintain it alive. There are two types of bacteriums: – anaerobic ( does non necessitating O for growing ) and aerophilic ( requires O for growing ) . Anaerobes usage glycolysis for energy, while aerobes use cellular respiration. ‘Oxygen can really be instead toxic, and for a cell to be able to utilize molecular O, it must be able to fabricate specific enzymes that detoxify oxygen waste merchandises, ‘ ( 1 ) .

Bacterias spread by spliting.

They can multiply at a rate of: – duplicating of all time 20min if the conditions are ideal. Most bacteriums prefer an optimum temperature of about 37i‚°C.

The temperature of the human organic structure is about 37i‚°C, and this is why bacteriums normally thrive on the human organic structure. Consequently it is of import to hold merchandises that will incorporate and destruct unneeded bacteriums.

hypertext transfer protocol: //www.medterms.com/script/main/art.asp? articlekey=13954

hypertext transfer protocol: //en.wikipedia.org/wiki/Anaerobic_organism

( 1 ) hypertext transfer protocol: //microbiology.suite101.com/article.cfm/difference_between_aerobic_anaerobic_bacteria ( Tami Port, 2009 )

hypertext transfer protocol: //users.rcn.com/jkimball.ma.ultranet/BiologyPages/E/Eubacteria.html

hypertext transfer protocol: //www.cellsalive.com/ecoli.htm

hypertext transfer protocol: //science.jrank.org/pages/714/Bacteria.html

Serratia marcescens

Serratia marcescens is a infective, anaerobiotic bacteria. Serratia Marcescens comes from the bacteriums household Enterobacteriaceae. The bacterium has a pH of 5-9, and can turn within temperatures of 5-40i‚°c but its optimum temperature ranges between 30-35i‚°c. Bing anaerobiotic it uses glycolysis for its energy. Glycolysis in the cytol quickly breaks down glucose molecules into two pyruvate ions ( pyruvic acid ) , which create two to four ATP molecules.

Serratia marcescens normally ranges between dark red to blanch pink in coloring material, as it has a pigment called prodigiosin. It has a cell form of B, and the gm discoloration is negative. Negative significance it has an extra outer membrane incorporating lipoids.

Figure 1 B form bacteriums

Serratia spreads by direct contact. The bacterium is normally associated with ; urinary piece of land infections, respiratory piece of land infections, meningitis and arthritis. Its natural home ground normally occurs in dirt H2O, and bowels.

hypertext transfer protocol: //www.bacteriamuseum.org/cms/Pathogenic-Bacteria/pathogenic-bacteria.html ( Dr. T. M. Wassenaar, 2009 )

Heinemann Queensland scientific discipline undertaking, Biology a contextual attack text book, ( Maggie spenceley, Barbra Weller, Margaret Mason, Katharine Fullerton, Chris Tsilemanis, Barbra Evans, Pauline Ladiges, John McKenzie, Phil Batterham, 2004 )

hypertext transfer protocol: //emedicine.medscape.com/article/228495-overview, ( Basilio J Ania, 2009 )

hypertext transfer protocol: //en.wikipedia.org/wiki/Serratia_marcescens, Hejazi A, Falkiner FR ( 1997 ) . “ Serratia marcescens ” . J Med Microbiol

www.sunysccc.edu/academic/mst/microbes/23smarc.htm

hypertext transfer protocol: //www.horizonpress.com/gateway/gram-negative-bacteria.html

Tea tree oil

History

Tea tree oil was foremost used by autochthonal people 1000s of old ages ago. They used the tea tree foliages by oppressing them and using them to lesions and cuts. They besides inhaled the oil for respiratory piece of land infections and to ease congestion.

Tea tree oil was discovered scientifically in 1923, by Arthur Penfold. The oil is obtained by the root distillment of foliages of Melaleuca aternfolia, a native works to Australia. Penfold tested the oil and discovered that its antiseptic belongingss were approximately 12 times stronger than carbolic acid, which was widely used.

During World War II tea tree oil was distributed to Australian soldiers in their first assistance kits, as it was considered as a close perfect antiseptic. Throughout the war the oil was acclaimed for its insect repellant and anti-fungal belongingss.

Tea tree oil has been tested by many Australian research workers and scientists, and has been proven to work better than some commercially available antibiotics.

hypertext transfer protocol: //vitanetonline.com/forums/1/thread/441, ( 2005 )

hypertext transfer protocol: //www.thursdayplantation.com/cmsv1/index.php? option=com_content & A ; task=view & A ; id=82ltemid=57

What it is effectual against and how it works

Tea tree oil is a pure natural antiseptic which contains ; a high terpinene-4-ol content, the primary germicidal active, and low Para-cymene content to understate skin annoyance. Tea tree oil plants as antiseptic, bactericide and fungicide incorporating 48 different organic compounds. It heals from within by perforating the cuticle. Some of the things people use this oil for include: jock ‘s pes, acne, furuncles, Burnss, warts, tonsillitis, fistula infections, tinea, tegument roseolas, impetigo, caput lice, insect repellant, cold sores, insect bites, and fungous infections. The oil works in different ways to accomplish the coveted consequence, such as with acne ; it disperses the Pus in the hickeies to clean and mend. Unlike other medicine for acne, it does non damage the tegument with side effects of dry tegument, biting, combustion, inflammation and frightening. Tea tree oil is able to kill bacteriums on the tegument, by piercing the beds of the tegument. One of the grounds tea tree oil is so effectual is because it is non widely used, and many other medicines are going resistant to bacteriums.

hypertext transfer protocol: //vitanetonline.com/forums/1/Thread/441, ( 2005 )

www.thenakedscientists.com/HTML/content/news/news/1079/ , ( 2001 )

Antiseptic pick

Antiseptic pick was designed to mend and forestall infections by suppressing or change by reversaling growing of bacteriums. Unlike antibiotics which are used to destruct bacteriums inside the organic structure, antiseptics are used to destruct bacteriums on the tegument and tissue. Antiseptics are used by using the pick to the tegument and either go forthing on to vaporize or rinse off after a certain period of clip. Antiseptic creamworks by changing the pH degree of the bacteriums, therefore the bacterium is destroyed as it ca n’t populate or turn.

hypertext transfer protocol: //scienceray.com/biology/antiseptics-and-bacteria/ # ixzz0m77KEBmr

Bepanthen Antiseptic Cream

Bepanthen pick is an antiseptic pick used for the intervention of scratchs, minor infections and is specifically used for crisp roseola. Bepanthen Antiseptic Cream contains: Prunus dulcis oil, dexpanthenol ( provitamin B5 ) and a soft antiseptic. Each gm contains Benzalkonium Chloride 500mcg.

Ingredients

The Benzalkonium Chloride solution in the Bepanthan antiseptic pick, is a Bactericide, Algaecide, and Fungicide, with a pH of 7.5. It contains three chemicals dwelling of ; biocide, a cationic wetting agent and stage transportation agent, and is soluble in ethyl alcohol and propanone. Benzalkonium is normally used for disinfecting tegument in readying for scratchs of panpipes.

Dexpanthenol is the D signifier of Panthenol, intending it is biologically active. ‘Panthenol is the alcohol signifier of pantothenic acid, more familiar as Vitamin B5. ‘ ( 2 ) . It helps better the healing of cuticular lesions, and besides hydrates, every bit good as cut downing itchiness and redness. Its chemical expression consists of: HO-CH2-C ( CH3 ) 2-CH ( OH ) -CONH-CH2CH2CH2-OH.

( 2 ) hypertext transfer protocol: //sci-toys.com/ingredients/panthenol.html

hypertext transfer protocol: //www.epharmacy.com.au/product.asp? id=1117 & A ; pname=Bepanthen+Antiseptic+Cream+100g

hypertext transfer protocol: //en.wikipedia.org/wiki/Benzalkonium_chloride- Benzalkonium Chloride Solutions ” . QuatChem Limited. 2009. hypertext transfer protocol: //www.quatchem.co.uk/55.html. Retrieved 2009-03-06.A

U.S. Environmental Protection Agency- Micheal Smith- April 1993

hypertext transfer protocol: //en.wikipedia.org/wiki/Panthenol

Negatives of antiseptic picks

There are a few grounds antiseptic picks do n’t work. Largely this is because people do non use adequate substance to the septic country. Another ground why it is sometimes non effectual is because the specific bacteria has developed a opposition to the merchandise. The overexploitation of antibacterial merchandises has really been proved to assist salvage bacteriums instead than killing them. ‘Bacteria signifier these unsusceptibilities when they are exposed to an antibacterial solution, but by some happenstance a few are n’t destroyed ‘ ( 3 ) . These lasting cells so pass on their unsusceptibility to other bacteriums through bacterial concurrence.

hypertext transfer protocol: //scienceray.com/biology/human-biology/antiseptics-and-bacteria/ # ixzz0m77KEBmr ( Aaron Boyle, 2008 )

Honey

Honey has the ability to mend as it has a natural H peroxide antibacterial belongings. After bee ‘s gather honey, to continue it they add an enzyme called glucose oxidase. ‘When honey comes into contact with organic structure moisture the glucose oxidase enzyme easy releases the antiseptic H peroxide. ‘ ( 4 ) . This H peroxide provides an antiseptic effectual on bacteriums, that is non tissue damaging.

hypertext transfer protocol: //manukahoney.com/resources/research/research01.html, ( 2003, Dr Peter Molan )

hypertext transfer protocol: //manukahoney.com/resources/research/secondagent.html

hypertext transfer protocol: //manukahoney.com/resources/umf.html

hypertext transfer protocol: //manukahoney.com/resources/research/whatisspecial.html

Factors impacting H peroxide in honey

Hydrogen peroxide in the honey can be affected by many factors. This is why it is non a sufficient antiseptic without UMF every bit good. Some of these factors include:

Some nectars destroy the glucose oxidase, because they produce a catalase that does this.

How it has been handled in processing, as glucose oxidase is easy destroyed by heat, fluid and sunshine.

Hydrogen peroxide can be broken down by a catalase enzyme in the organic structure tissue and serum, therefore cut downing its effectivity when used on an infection or lesion.

Does non trip if there is non moisture, but will be destroyed if there is excessively much wet.

Glucose oxidase requires O.

hypertext transfer protocol: //manukahoney.com/resources/research/research01.html, ( 2003, Dr Peter Molan )

UMF Manuka Honey

Manuka honey is gathered from the Manuka flowers ( Leptospermum scoparium ) . There are two types of Manuka honey. The ordinary Manuka honey which merely contains hydrogen peroxide, and UMF Manuka honey which contains its ain antibacterial belongings UMF. UMF ( alone Manuka Factor ) is found in some strands of Manuka honey, and is a phyrochemical belongings. UMF is a non-peroxide bactericide, that is said to hold been effectual against a scope of bacteriums including ; Staphylococcus aureus ( cause of septic lesions, and MRSA ) , streptococcus pyogenes ( cause of sore pharynxs ) , and helicobacter pylori ( bacteriums of most stomach ulcers ) . The UMF evaluation refers to the antibacterial belongingss found within the Manuka honey. The UMF evaluation is placed on every packaged honey with a UMF degree over 10. To prove these degrees each single batch of honey is tested, as degrees of UMF vary easy as UMF is non found within every Manuka works. The degrees of UMF are considered ; 10-15: utile degrees to cover with bacteriums, 16-30: superior degrees of high activity, effectual in handling bacteriums.

hypertext transfer protocol: //manukahoney.com/resources/research/research01.html, ( 2003, Dr Peter Molan )

hypertext transfer protocol: //manukahoney.com/resources/research/secondagent.html

hypertext transfer protocol: //manukahoney.com/resources/umf.html

hypertext transfer protocol: //manukahoney.com/resources/research/whatisspecial.html, ( 2003, SummerGlow Apiaries )

hypertext transfer protocol: //www.thehoneyfarm.com.au/acatalog/How_Does_it_Work_.html

Positives of UMF Manuka Honey

UMF Manuka Honey has been proven to hold significantly more effectual antibacterial merchandise than normal honey ( incorporating merely hydrogen peroxide ) . UMF works by perforating deep into skin tissue, making deep into infections. ‘It has been found able to perforate 1cm of porc tegument, fat and musculus overnight. ‘ ( 5 ) .Once in the septic country it has the ability to clean lesions by raising soil and bacteriums out of the lesion bed through osmosis. Unlike H peroxide, UMF does non necessitate O, so it can be smothered by wound dressings or pits and still accomplish the antibacterial consequence required. The honey draws foods and organic structure fluids to the lesion, cut downing scarring and helping in cell growing. UMF is besides an anti-inflammatory, as it contains antioxidants, it can wipe up up destructive free groups, and this besides helps help in hurting alleviation. The pH of this honey is really low, so it has the ability to decelerate down and prevent growing of bacteriums because of its sourness. Unlike H peroxide, UMF is really stable. It is immune to heat, fluid, sunshine, and is non broken down by the accelerator in the organic structure. There is besides a batch of grounds back uping that together, H peroxide and UMF has a interactive consequence. Meaning the combination of these two merchandises has a greater consequence when together, hence doing honey more effectual as an antiseptic.

hypertext transfer protocol: //manukahoney.com/resources/research/whatisspecial.html, ( 2003, SummerGlow Apiaries )

hypertext transfer protocol: //manukahoney.com/resources/research/research01.html

hypertext transfer protocol: //manukahoney.com/resources/research/secondagent.html

hypertext transfer protocol: //manukahoney.com/resources/umf.html

hypertext transfer protocol: //www.thehoneyfarm.com.au/acatalog/How_Does_it_Work_.html

hypertext transfer protocol: //manukahoney.com/resources/research/wound-care.html

Hypothesis

Natural and semisynthetic antiseptics will hold the same effects on the bacteria civilization ‘Serratia marcescens. ‘

Apparatus

Petriy Dishes ( 6 )

Serratia Marcens ( Bacteria Culture )

Incubator

Parafilm

Aga Gel

Cotton Buds- Sterilized

forceps

Internet ( research )

Detergent spray to clean country after experiment

Camera

Baseball gloves

Protective spectacless

Ruler

Pens/pencils

Method

Part A: making bacteriums civilization

Recover the needed sum of petri dishes incorporating agar gel from the electric refrigerator

Paint the dishes really lightly utilizing sterilised cotton buds, utilizing bacteriums civilization ( serratia marcescens ) , foremost across four ways so round the border, doing certain the dish had a generous sum of genus Serratia marcescens.

Seal dishes with paraphilm, leting air through but non bacteriums.

repeated for all of the dishes

Then place the pertri dishes in an brooder, set to 30i‚°c. Leave to sit there for 4 yearss.

Part B: Experiment

Retrieve required sum of petri dishes from the brooder 4 yearss after being placed in at that place.

Using paper and a hole cowboy, punch out four holes for each petri dish ( used for a stopper in the experiment, leting to command the sum of solution applied onto the bacteria civilization ) .

Put the different solutions in separate dishes.

The forceps so necessitate to be sterilised: foremost dipped in intoxicant, so flamed over a bluish fire utilizing Bunsen burners.

Using the sterilized forceps, one stopper ( hole punched paper ) is so dipped in the first solution ( e.g. tea tree oil ) , and so placed in the petri dish, doing certain that it is far plenty off from the border

Between plugs the forceps must be re-sterilised for the demand to consistency/ cross taint.

This procedure is repeated for the other 3 stoppers, doing certain that they are an equal distance apart within the dish.

This is so repeated for the other solutions ( e.g. antiseptic pick, and Manuka honey ) .

Once the petri dishes are complete they need to be re-sealed with paraphilm and so placed in the brooder at 30i‚°C.

Consequences

Thursday 20/5/10

The honey had the most consequence on the bacteria civilization, with the country of consequence diameter of 1cm ( diameter ) , after being in the brooder for 3 yearss.

Figure 2 Manuka Honey

The antiseptic pick had a minimum consequence, with the scope of merely 0.6cm ( diameter ) . The country of consequence merely merely reached outside of the country of the stopper.

Figure 3 Antiseptic pick

The tea tree oil appeared to do no consequence on the bacteria civilization, with its scope of 0.55cm ( diameter ) .

Figure 4 Tea tree oil

Monday 24/5/10

The UMF Manuka honey 30+ had the greatest consequence on the Serratia marcescens. The 30+ UMF had an country of consequence of 1.3cm ( diameter ) , after being in the brooder for 3 yearss.

The Manuka honey active 10+ had an country of consequence of 1cm ( diameter ) . The 10+ honey besides had another ring of consequence on the bacterium which ranged 1.5cm off from the Centre of the stopper with a diameter of 3cm.

The normal table honey made an country of impact of 0.7cm ( diameter ) . It besides had a big circle of consequence ( diameter of 2.5cm ) , where the honey had dispersed but non killed all of the bacteriums.

The merchandises ( tea tree oil, antiseptic pick, and Manuka honey ) that were put in the brooder a hebdomad ago were besides observed. There was no alterations in the antibacterial consequence from 20/5/10.

Discussion

Cite this Natural Antiseptics Vs Medical Antiseptics Biology

Natural Antiseptics Vs Medical Antiseptics Biology. (2017, Jul 15). Retrieved from https://graduateway.com/natural-antiseptics-vs-medical-antiseptics-biology-essay/

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