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Extraction Of Essential Oil Using Steam Distillation Biology

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Admiting about the importance of workss and its medicative value research work is being done on the works stuff. There are besides over 200 mentions to aromatics, incense and unctions in the Old and New Testaments ; Frankincense, Myrrh, Galbanum, Cinnamon, Cassia, Rosemary, Hyssop and Spikenard are noted for being used for anointing rites and healing of the ill. Research has confirmed centuries of practical usage of indispensable oils, and we now know that the ‘fragrant pharmaceutics ‘ contains compounds with an highly wide scope of biochemical effects.

The oils usually bear the name of the works species from which they are derived. Essential oils are so termed as they are believed to stand for the really kernel of olfactory property and spirit. The recovery of indispensable oil ( the value added merchandise ) from the natural botanical get downing stuff is really of import since the quality of the oil is greatly influenced during this measure. There are a assortment of methods for obtaining volatile oils from workss.

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Steam distillment method is found to be one of the promising techniques for the extraction of indispensable oil from workss as reputable distiller will continue the original qualities of the works and is being done in Clevenger setup. Analysis of Essential oil is done Gas Chromatography which gives us some indicants of the quality and genuineness of the oil.

Chapter 1



Essential oils contain extremely volatile substances that are isolated by a physical method or procedure from workss of a individual botanical species. The oils usually bear the name of the works species from which they are derived. Essential oils are so termed as they are believed to stand for the really kernel of olfactory property and spirit. Essential oil workss and culinary herbs include a wide scope of works species that are used for their aromatic value as flavorers in nutrients and drinks and as aromas in pharmaceutical and industrial merchandises. Essential oils derive from aromatic workss of many genera distributed worldwide.

Oils are used in the embalming procedure, in medical specialty and in purification rites. There are besides over 200 mentions to aromatics, incense and unctions in the Old and New Testaments. Research has confirmed centuries of practical usage of Essential Oils, and we now know that the ‘fragrant pharmaceutics ‘ contains compounds with an highly wide scope of biochemical effects. There are about three hundred indispensable oils in general usage today by professional practicians. Continual barrage of viral, bacterial, parasitic and fungous taint occurs in our organic structure. Essential oils are a great benefit to assist protect our organic structures and places from this onslaught of pathogens. Immune system needs support and these indispensable oils can give the needed indorsement. [ 1 ]

Steam distillment is used in the extraction of Essential Oil from the works stuff. It is a particular type of distillment or a separation procedure for temperature sensitive stuffs like oils, rosins, hydrocarbons, etc. which are indissoluble in H2O and may break up at their boiling point. The cardinal nature of steam distillment is that it enables a compound or mixture of compounds to be distilled at a temperature well below that of the boiling point ( s ) of the single component ( s ) . Essential Oil contains constituents with boiling points up to 200A°C or higher temperatures. In the presence of steam or boiling H2O, nevertheless, these substances are volatilized at a temperature near to 100A°C, at atmospheric force per unit area. [ 2 ]

Analysis of Essential Oil is done by utilizing Gas Chromatography with Mass Spectrometer. The qualitative and quantitative analysis is done to cognize the components in the oil and the per centum of constituents present in the oil severally, by making so we can cognize the pureness of that peculiar oil. [ 3 ]

Chapter 2


It is estimated that there are 250,000 to 500,000 species of workss on Earth. A comparatively little per centum ( 1 to 10 % ) of these is used as nutrients by both worlds and other carnal species. It is possible that even more are used for medicative intents ( Moerman, D. E. 1996 ) . Moerman ( 1996 ) reported that while 625 species of workss have been used by assorted Native American groups as nutrient, 2,564 have found usage as drugs. Harmonizing to his computations, this leaves about 18,000 species of workss which were used for neither nutrient nor drugs. [ 4 ]

Plant oils and infusions have been used for a broad assortment of intents for many 1000s of old ages ( Jones 1996 ) . These intents vary from the usage of rosewood and cedar wood in perfumery, to seasoning drinks with calcium hydroxide, fennel or retem berry oil, and the application of lemon grass oil for the saving of stored nutrient harvests. In peculiar, the antimicrobic activity of works oils and infusions has formed the footing of many applications, including natural and processed nutrient saving, pharmaceuticals, alternate medical specialty and natural therapies.

Since antediluvian times, herbs and their indispensable oils have been known for their varying grades of antimicrobic activity. More late, medicative works infusions were developed and proposed for usage in nutrient as natural disinfectants.

2.1 Definition:

An indispensable oil is a concentrated, hydrophobic liquid incorporating volatile olfactory property compounds from workss. Essential oils are besides known as volatile, aeriform oils or aetherolea, or merely as the “ oil of ” the works from which they were extracted, such as oil of clove. Oil is “ indispensable ” in the sense that it carries a typical aroma, or kernel, of the works. [ 5 ]

Essential oils are often referred to as the “ life force ” of workss. These “ indispensable ” oils are extracted from flowers, foliages, stems, roots, seeds, bark, and fruit rinds. The sum of indispensable oils found in these workss can be anyplace from 0.01 per centum to 10 per centum of the sum. These oils have powerful antimicrobic factors, holding broad scope of curative components. These oils are frequently used for their spirit and their curative or odoriferous belongingss, in a broad choice of merchandises such as nutrients, medical specialty, and cosmetics. Merely pure oils contain a full spectrum of compounds that cheap imitations merely can non double. [ 5 ]


Major Raw Material Used In Extraction of Essential Oils:







Bay foliage



Lemon Grass








Tea Tree




Clary Sage












Grape fruit


Calcium hydroxide







Nutmeg Oil


















2.2 CHEMICAL CONSTITUENTS OF ESSENTIAL OILS: An Essential Oil contains more than 200 chemical constituents, but some are many times more complex. Essential oils consist of chemical compounds which have H, C and O as their edifice blocks. They can be basically classified into two groups:

Volatile fraction: Essential oil constituting of 90-95 % of the oil in weight, incorporating the monoterpene and sesquiterpene hydrocarbons, every bit good as their oxygenated derived functions along with aliphatic aldehydes, intoxicants, and esters.

Nonvolatile residue: This comprises 1-10 % of the oil, incorporating hydrocarbons, fatty acids, steroid alcohols, carotenoids, waxes, and flavonoids.

However the belongingss of these constituents can alter. For illustration, the constituents from the oils extracted from workss can alter harmonizing to how, when and where these workss are grown and harvested. [ 6 ] The components can be once more subdivided into 2 groups, such as the hydrocarbons which are made up of largely terpenes and the oxygenated compounds which are chiefly alcohols, aldehydes, esters, ketones, phenols and oxides. Some of the common constituents are listed below along with their belongingss.


Alcohols are by and large considered safe and have a really low or wholly absent toxic reaction in the organic structure or on the tegument and so can be used on kids. They are highly utile due to their antiviral, antibacterial and antiseptic belongingss. Alcohols are present either as a free compound or combined with a terpene or ester and are found in ylang -ylang and lavender as linalool, geraniol in geranium and palmarosa and citronellol found in rose, lemon and eucalyptus. Other alcohols include menthol, nerol and benzyl intoxicant.


Alderhydes are found in lemon-scented oils such as Melissa, lemon vervain, citronella etc. and include citral, citronellal and neral. They by and large have ataractic qualities with specific antiseptic belongingss. Other known alderhydes include benzaldehyde, cinnamic alderhyde and perillaldehyde. Essential oils incorporating alderhydes are helpful in handling redness, Candida and viral infections. [ 6 ]


Constructing blocks of Essential Oil are H and C. Basic Hydrocarbon found in workss is isoprene holding the undermentioned construction.

( Isoprene )


These constituents by and large have names stoping with “ east northeast ” . Some of them are limonene, pinene, piperene, camphene etc. These constituents act as an antibacterial, antiviral, anti-inflammatory, antiseptic, antiviral and disinfectant. These are farther categorized into monoterpene, sesquiterpene and diterpenes. When two of the isoprene units are joined caput to chase, the consequence is a monoterpene, when three are joined, it ‘s a sesquiterpene and likewise four linked isoprene units are diterpenes.

Monoterpene [ C10H16 ] :

Monoterpene are of course happening compounds, the bulk being unsaturated hydrocarbons ( C10 ) .But some of their oxygenated derived functions such as intoxicants, Ketones, and carboxylic acids known as monoterpenoids.

( Limonene ) ( Menthol )

Two isoprene units are present in these branched-chain C10 hydrocarbons and are widely distributed in nature with more than 400 of course happening monoterpenes. Furthermore, besides being additive derived functions ( Geraniol, Citronellol ) , the monoterpenes can be cyclic molecules ( Menthol – Monocyclic ; Camphor – bicyclic ; Pinenes ( I± and I? ) – Pine genera every bit good. Thujone ( a monoterpene ) is the toxic agent found in Artemisia absinthium ( wormwood ) from which the cordial common wormwood, is made. Borneol and camphor are two common monoterpenes. Borneol, derived from pine oil is used as a germicide and deodourant. Camphor is used as a counterirritant, anaesthetic, expectorant, and antipruritic, among many other utilizations.


Sesquiterpenes are biogenetically derived from farensyl pyrophosphate and in construction may be additive, monocyclic or bicyclic. They constitute a really big group of secondary metabolites, some holding been shown to be stress compounds formed as a consequence of disease or hurt. These are holding belongingss like anti-inflammatory, anti-septic, analgetic and anti-allergic.

Sesquiterpene Lactones:

These are available as farnesene in camomile and lavender. They non merely have proved to be of involvement from chemical and chemotaxonomic point of position, but besides possess many antitumor, anti-leukemia, cytotoxic and antimicrobic activities.

Chemically the compounds can be classified harmonizing to their carboxylic skeletons ; therefore, guaianolides, pseudoguaianolides, eudesmanolides, eremophilanolides, xanthanolides, etc. can be derived from the germacranolides.

Structural characteristics of all these compounds are associated with much of the biological activity. For illustration beta-caryophyllene in basil and black Piper nigrum


Isoprene has been an built-in portion in most of the constituents as there are four isoprene units in Diterpenes. By Stem Distillation method we can non observe Diterpenes as this molecule is excessively heavy to let for vaporization, so it is seldom found in distilled Essential Oils. Diterpenes occur in all works households and consist of compounds holding a C20 skeleton. There are about 2500 known Diterpenes that belong to 20 major structural types. Derived functions of Diterpenes are works endocrines Gibberellins and phytol happening as a side concatenation on chlorophyll. The biogenesis occurs in plastids and interestingly mixtures of monoterpenes and diterpenes are the major components of works rosins. In a similar mode to monoterpenes, Diterpenes arise from metamorphosis of geranyl geranyl pyrophosphate ( GGPP ) . Therapeutically Diterpenes have limited importance and are used in certain depressants ( coughs ) every bit good as in spasmolytics and minor tranquilizers.


Naturally Alcohols exist either as a free compound or combined with a terpenes or ester. When terpenes are attached to an O atom, and H atom, the consequence is an intoxicant. When the terpene is monoterpene, the ensuing intoxicant is called a monoterpenol. Alcohols are non and are suited to organic structure or tegument. Therefore, they are considered safe to utilize. Some of these belongingss are anti-septic, anti-viral, disinfectant and germicidal. Some of the illustrations are linalool found in ilang-ilang and lavender, geraniol in geranium and rose and nerol in neroli.


Aldehyde incorporating Essential Oils are effectual in handling candida and other fungous infections. Some of these belongingss are anti-fungal, anti-inflammatory, anti-septic, anti-viral, disinfectant, bactericidal, and ataractic. Aldehydes are present as citral in lemon, Citronellal in lemon grass, lemon balm and citrous fruit eucalyptus.


By and large Organic acids are found in really little measures in their free province within Essential Oils. Plant acids act as constituents or buffer systems to command sourness. These besides act anti-inflammatory. Examples are cinnamic and benzoic acid in benjamin, Citric and lactic.


Esters are formed through the reaction of intoxicants with acids. Essential oils incorporating esters are used for their soothing, equilibrating effects. Because of the presence of intoxicant, they are effectual antimicrobic agents. Medicinally, esters are characterized as fungicide and ataractic, with a equilibrating action on the nervous system. They by and large are free from safeguards with the exclusion of methyl salicylate found in birch and pyrola which is toxic within the system. Examples are linlyl ethanoate in Citrus bergamia and lavender and Geranyl formate in geranium.


Ketones found in workss are used for upper respiratory ailments. They assist the flow of mucous secretion and easiness congestion. Essential oils incorporating ketones are good for advancing lesion healing and promoting the formation of cicatrix tissue. Ketones are ( non ever ) really toxic. The most toxic ketone is Thujone found in mugwort, sage, golden buttons, Thuja and wormwood oils. Other toxic ketones found in indispensable oils are pulegone in Mentha pulegium, and pinocamphone in Hyssopus officinaliss. Some non-toxic ketones are jasmone in jasmine oil, fenchone in fennel oil, carvone in Mentha spicata and dill oil and menthone in Mentha piperita oil.


Lactones are known to be peculiarly effectual for their anti-inflammatory action, perchance by their function in the decrease of prostaglandin synthesis and expectorant actions. Lactones have an even stronger expectorant action than ketones. [ Previous thesis ]


AROMATHERAPHY: Aromatherapy is a signifier of alternate medical specialty that uses volatile works stuffs, known as indispensable oils, and other aromatic compounds for the intent of changing a individual ‘s temper, cognitive map or wellness. Science has discovered that our sense of odor plays a important function in our overall wellness.

Since ancient times Essential Oils have been used in medical specialty because of their medicative belongingss, for illustration some oils have antiseptic belongingss. In add-on, many have an uplifting consequence on the head, though different indispensable oils have different belongingss.

Working of Essential Oil in Aromatherapy: when Essential Oil is inhaled it goes straight from olfactory system to limbic system of the encephalon. Brain responds to the peculiar aroma impacting our emotions and chemical balance. Essential Oils besides absorbed by the tegument and carried throughout the organic structure via the circulatory system to make all internal variety meats.

We can be benefited by taking carefully the desired and suited oils which can advance overall wellness. Benefits depend upon the alone nature of each individual ‘s response to an aromatic stimulation. [ 1 ]

Importance of Essential Oil in pharmacies: Essential Oils have various applications in pharmacies. Some of the applications are listed below.

Antiseptics: The antiseptic belongingss of Essential Oil make them active against broad scope of bacteriums as on antibiotic resistant strains. In add-on to this they are besides against Fungis and barms. The most common beginnings of indispensable oils used as antiseptics are: Cinnamon, Thyme, Clover, Eucalyptus, Culin savory, Lavender. Citral, geraniol, linalool and thyme camphor are much more powerful than phenol. [ 1 ]

Expectorants and water pills: When used externally, indispensable oils like ( L’essence de terebenthine ) addition microcirculation and supply a little local anaesthetic action. Till now, indispensable oils are used in a figure of unctions, pick and gels, whereby they are known to be really effectual in alleviating sprains and other articular strivings. Oral disposal of indispensable oils like eucalyptus or pin oils, stimulate ciliated epithelial cells to release mucous secretion. On the nephritic system, these are known to increase vasodilation and in effect bring about a diuretic consequence.

Spasmolytic and ataractic: Essential oils from the Umbellifereae household, Mentha species and vervains are reputed to diminish or extinguish GI cramps. These indispensable oils increase secernment of stomachic juices. In other instances, they are known to be effectual against insomnia.

METHODS OF EXTRACTION: The following are the methods of extraction of Essential Oil and their drawbacks.

2.4.1 Solvent-Extraction:

In the Solvent-Extraction method of Essential Oils recovery, an pull outing unit is loaded with pierced trays of indispensable oil works stuff and repeatedly washed with the dissolver. A hydrocarbon dissolver is used for extraction. All the extractible stuff from the works is dissolved in the dissolver. This includes extremely volatile olfactory property molecules every bit good as non-aroma waxes and pigments. The infusion is distilled to retrieve the dissolver for future usage. The waxen mass that remains is known as the concrete. The concentrated concretes are farther processed to take the waxy stuffs which dilute the pure indispensable oil. To fix the absolute from the concrete, the waxy concrete is warmed and stirred with intoxicant ( ethyl alcohol ) . During the warming and stirring procedure the concrete breaks up into minute globules. Since the olfactory property molecules are more soluble in intoxicant than the waxes, an efficient separation of the two consequences. This is non considered the best method for extraction as the dissolvers can go forth a little sum of residue behind which could do allergic reactions and consequence the immune system.

2.4.2 Maceration:

Maceration really creates more of “ infused oil ” instead than an Essential Oil. Plant affair is soaked in vegetable oil, heated and strained at which point it can be used for massage. This method is non desirable because it changes the composing of oil.

2.4.2 Cold Press:

This method is used to pull out the Essential Oils from citrous fruit rinds such as orange, lemon, Citrus paradisi and Citrus bergamia. This method involves the simple pressure of the rind at about 120 grades F to pull out the oil. The rinds are separated from the fruit, are land or chopped and are so pressed. The consequence is a watery mixture of indispensable oil and liquid which will divide given clip. Small change from the oil ‘s original province occurs – these citrous fruit oils retain their bright, fresh, uplifting olfactory properties like that of smelling a wondrous mature fruit. The drawback of this method is, oils extracted utilizing this method have a comparatively short shelf life.

2.4.3 Effleurage:

This is one of the traditional ways of pull outing oil from flowers. The procedure involves layering fat over the flower petals. After the fat has absorbed the indispensable oils, intoxicant is used to divide and pull out the oils from the fat. The intoxicant is so evaporated and the Essential Oil is collected.

2.4.4 Super Critical CO2 Extraction:

Supercritical CO2 extraction ( SCO2 ) involves C dioxide heated to 87 grades F and pumped through the works stuff at around 8,000 pounds per square inch, under these conditions ; the C dioxide is likened to a ‘dense fog ‘ or vapours. With release of the force per unit area in either procedure, the C dioxide escapes in its gaseous signifier, go forthing the Essential Oil buttocks. The usual method of extraction is through steam distillment. After extraction, the belongingss of a good quality indispensable oil should be every bit near as possible to the “ kernel ” of the original works. The key to a ‘good ‘ indispensable oil is through low force per unit area and low temperature processing. High temperatures, rapid processing and the usage of dissolvers alter the molecular construction, will destruct the curative value and change the aroma.

2.4.5 Turbo Distillation Extraction:

Turbo distillment is suited for hard-to-extract or harsh works stuff, such as bark, roots, and seeds. In this procedure, the workss soak in H2O and steam is circulated through this works and H2O mixture. Throughout the full procedure, the same H2O is continually recycled through the works stuff. This method allows faster extraction of indispensable oils from hard-to-extract works stuffs.

Dr.A.Sahoo et Als have studied that Steam Distillation is a particular type of distillment or a separation procedure for temperature sensitive stuffs like oils, rosins, hydrocarbons, etc. which are indissoluble in H2O and may break up at their boiling point. The temperature of the steam must be high plenty to zap the oil nowadays, yet non so high that it destroys the workss or burns the indispensable oils. The experiment has been carried out for the extraction of oil from Eucalyptus which has high indispensable oil content. Such Eucalyptus indispensable oil, which have been used as aroma and chemical natural stuffs for a long clip, are now been studied as renewable beginnings of energy.

G. Anitescu et Als have studied that mature fruits of Coriander sativum L. were extracted by steam distillment and by supercritical fluid extraction ( SFE ) , utilizing CO2 in a two-stage separation system. An cheap thermic enlargement process for supercritical fluid bringing has been developed. The designation of constituents was performed by gas chromatography and mass spectroscopy ( GCA±MS ) . The per centum composing of the 40 identified compounds was compared with the composing of commercial Chinese parsley oil extracted by hydro distillment.

Roy Teranishi et Als have studied that system combines steam distillment and liquid-liquid extraction to retrieve volatiles from fats and oils. Oil is pumped in at the top of a spinning-band distillment column, in which the oil is heated to100 “ C and spread to a thin movie. As the oil movie drops down to the pot, steam, which is introduced at the underside, travels upward to deprive the volatiles from the oil. The steam distillation is extracted in a liquid-liquid extractor incorporated in the system, and the extracted H2O is recycled as steam. Stripped oil in the pot serves as a liquid seal to coerce steam up the column. The degree of the oil in the pot is maintained automatically by an overflow system. Many litres of oil can be pumped through this system to be stripped of volatiles by steam. The volatiles can be isolated easy from the little sum of dissolver recycled in the liquid-liquid extractor.

Mentioning to the above literature reappraisal, it was found that Steam Distillation method is an appropriate and economical method for extraction of Essential Oil.

2.4.6 Extraction of Essential Oils Using Steam distillment Method:

Steam distillment is a particular type of distillment or a separation procedure for temperature sensitive stuffs like oils, rosins, hydrocarbons, etc. which are indissoluble in H2O and may break up at their boiling point. The cardinal nature of steam distillment is that it enables a compound or mixture of compounds to be distilled at a temperature well below that of the boiling point ( s ) of the single component ( s ) . Essential oils contain substances with boiling points up to 200A°C or higher temperatures. In the presence of steam or boiling H2O, nevertheless, these substances are volatilized at a temperature near to 100A°C, at atmospheric force per unit area.

Fresh, or sometimes dried, botanical stuff is placed in the works chamber of the still and the steam is allows to go through through the herb stuff under force per unit area which softens the cells and allows the Essential Oil to get away in vapor signifier. The temperature of the steam must be high plenty to zap the oil nowadays, yet non so high that it destroys the workss or burns the Essential Oils.A Besides the steam bantam droplets of Essential Oil evaporates and travel through a tubing into the still ‘s condensation chamber. Here Essential Oil bluess condense with the steam.A The indispensable oil signifiers a movie on the surface of the water.A To divide the Essential Oil from the H2O, the movie is so decanted or skimmed off the top.A The staying H2O, a by-product of distillment, is called flowered H2O, distillation, or hydrosol. It retains many of the curative belongingss of the works, doing it valuable in tegument attention for facial mists and toners ( A solution incorporating chemicals that can alter the colour of a photographicA print ) .A In certain state of affairss, flowered H2O may be preferred to be pure indispensable oil, such as when handling a sensitive person or a kid, or when a more diluted intervention is required. Rose hydrosol, for illustration, is normally used for its mild antiseptic and comforting belongingss, every bit good as its delighting flowered olfactory property.

A figure of factors determine the concluding quality of a steam distilled indispensable oil. Apart from the works stuff, most of import are clip, temperature and force per unit area, and the quality of the distillment equipment. Essential oils are really complex merchandises. Each is made up of many, sometimes 100s, of distinguishable molecules which come together to organize the oil ‘s olfactory property and curative belongingss. Some of these molecules are reasonably delicate constructions which can be altered or destroyed by inauspicious environmental conditions. So, much like a all right repast is more flavourful when made with forbearance, most oils benefit from a long, slow ‘cooking ‘ procedure. It is possible that longer distillment times may give more complete oil. It is besides possible nevertheless, that longer distillment clip may take to the accretion of more artefacts than normal. This may hold a funny consequence of looking to bettering the olfactory property, as sometimes when stuffs that have a larger figure of constituents are sniffed, the perceptual experience is frequently of somewhat increased edification, added comprehensiveness and character, and perchance, and excess pleasantness.

Advantages of utilizing Steam Distillation:

The advantage of Steam Distillation is that it is a comparatively inexpensive procedure to run at a basic degree, and the belongingss of oils produced by this method are non altered. As steam reduces the boiling point of a peculiar constituent of the oil, it ne’er decomposes in this method. This method apart from being economical, it is besides comparatively faster than other methods.

Chapter 3


3.1 Experimental Apparatus:

The conventional diagram of experimental apparatus is shown below. The experiment was conducted in a Clevenger ‘s Apparatus. Apparatus consist of one unit of ammunition underside flask of 1000ml which is connected with another two manner unit of ammunition flask which holds natural stuff. The top flask is connected with capacitor through the connection. The separating funnel is used for the separation of indispensable oil and H2O.

3.2 Experimental Procedure:

Fresh foliages ( rosemary ) or bark ( cinnamon ) are cut into pieces less than 2 Ten 2 centimeter within half a twenty-four hours after aggregation and 150-200 g boiled with 500 milliliter of distilled H2O in a Clevenger setup until oil distillment ceased after 5-6 h. The volume of indispensable oils was determined from a graduated trap. The indispensable oils in the distillation were dried over anhydrous Na2SO4 and kept in the deep-freeze.

Figure-1 Essential Oil Steam Distiller

Figure-2 Flow sheet of Steam Distillation

3.3 Analysis of Essential Oils:

Analysis of Essential Oil was done utilizing Gas Chromatography with Mass spectrometer to cognize the composing of oil and to the measure of each composing.

3.3.1 Gas Chromatography-Mass Spectrometer:

Gas Chromatography-Mass Spectrometry ( GC-MS ) is a method that combines the characteristics of gas-liquid chromatography and mass spectroscopy to place different substances within a trial sample. Applications of GC-MS include drug sensing, fire probe, environmental analysis, explosives probe, and designation of unknown samples. Additionally, it can place trace elements in stuffs that were antecedently thought to hold disintegrated beyond designation. [ 7 ]


The GC-MS is composed of two major constructing blocks: the gas chromatograph and the mass spectrometer. The gas chromatograph utilizes a capillary column which depends on the column ‘s dimensions ( length, diameter, movie thickness ) every bit good as the stage belongingss ( e.g. 5 % phenyl polysiloxane ) . The difference in the chemical belongingss between different molecules in a mixture will divide the molecules as the sample travels the length of the column. The molecules take different sums of clip ( called the keeping clip ) to come out of ( elute from ) the gas chromatograph, and this allows the mass spectrometer downstream to gaining control, ionise, speed up, debar, and observe the ionised molecules individually. The mass spectrometer does this by interrupting each molecule into ionised fragments and observing these fragments utilizing their mass to bear down ratio. These two constituents, used together, let a much finer grade of substance designation than either unit used individually. It is non possible to do an accurate designation of a peculiar molecule by gas chromatography or mass spectroscopy entirely. The mass spectroscopy procedure usually requires a really pure sample while gas chromatography utilizing a traditional sensor ( e.g. Flame Ionization Detector ) detects multiple molecules that happen to take the same sum of clip to go through the column ( i.e. have the same keeping clip ) which consequences in two or more molecules to co-elute. Sometimes two different molecules can besides hold a similar form of ionised fragments in a mass spectrometer ( mass spectrum ) . Uniting the two procedures makes it highly improbable that two different molecules will act in the same manner in both a gas chromatograph and a mass spectrometer. Therefore when an placing mass spectrum appears at a characteristic keeping clip in a GC-MS analysis, it typically lends to increased certainty that the analyte of involvement is in the sample. [ 7 ]

3.4 Experimental Observation:

Experiment had been conducted utilizing different works stuffs at different temperatures and clip of warming, maintaining force per unit area invariable ( atmospheric force per unit area ) .

2. Table demoing different volumes of Essential Oil extracted from different works stuffs and their clip of warming.

Sl. No.



Weight ( gram. )

Volume of distilled H2O ( milliliter )

Time of warming ( min )

Temperature ( 0C )

Volume of Essential Oil ( milliliter )

Volume of hydrosol ( milliliter )


Orange Peels






& gt ; 10







& lt ; 10














& gt ; 10







& gt ; 10


Eucalyptus foliages






& gt ; 10







& gt ; 10







& gt ; 10







& gt ; 10


Lemon foliages






& gt ; 10







& gt ; 10







& gt ; 10







& gt ; 10


Marigold flowers






& gt ; 10







& gt ; 10


Rose petals






& gt ; 10


Hibiscus foliages






& gt ; 10


Curry foliages






& gt ; 10

Chapter 4


4.1 Inferences:

Volume of Essential Oil obtained from a peculiar works stuff was different for different temperatures and at a peculiar temperature for different clip of heating. Volume of Essential Oil obtained is less in comparing to hydrosol of the same works stuff. Its fluctuation along with temperature and clip of warming is shown in graphs drawn below.

4.1.1 Graph demoing fluctuation of Volume of Essential Oil against Temperature, obtained from Orange Peels.

From the graph we can detect that volume of Essential Oil obtained from orange Peels is about increasing linearly with Temperature, if we ignore the little diminution in volume at 900C. The diminution may be due to leakage of vapour during Steam Distillation or because of improper decantation.

4.1.2 Graph demoing fluctuation of Volume of Essential Oil against Temperature, obtained from Eucalyptus foliages.

From the above graph we can detect that volume of Essential Oil obtained is negligible before the constituents of the oil range to their boiling point. After making to their boiling points Essential Oil is obtained and further we can theorize that if addition more temperature proliferation in volume can be achieved.

Now fluctuation of Volume of Essential Oil obtained with Time of heating for different works stuffs is shown below.

4.1.3 Graph demoing fluctuation of Volume of Essential Oil with Time of warming, obtained from Orange Peels.

From the graph we can detect that volume of Essential Oil obtained from orange Peels is about increasing linearly with clip of heating at a peculiar temperature ( 900C ) . But the increase is non steep in this instance.

4.1.4 Graph demoing fluctuation of Volume of Essential Oil against Time of warming, obtained from Lemon foliages.

From the graph we can detect that volume of Essential Oil obtained from Lemon leaves is about increasing linearly with clip of heating at a peculiar temperature ( 1000C ) . But the increase is non steep in this instance.

In instance of other works stuffs volume of Essential Oil was non obtained, may be due to the temperature supplied was non sufficient to zap the constituents of oils to their boiling points, as show in the tabular array 3.4.1.

4.2 Graphs demoing analysis of Essential Oil:

Analysis of Essential Oil obtained after Steam Distillation was done utilizing Gas chromatography-Mass Spectrometer method. The analysis shows the composing of the oil and besides the per centum of each constituent. This is shown in graph produced by Gas chromatography-Mass Spectrometer setup. The graph generated by Gas chromatography shows the composing of the oil and the other graph shown by Mass Spectrometer gives the per centum of each constituent. Some of the graphs obtained after analysis are shown below.

4.2.1 Graphs demoing the quantitative and qualitative analysis of Essential Oil extracted from Lemon foliages.

4.2.2 Graphs demoing the quantitative and qualitative analysis of Essential Oil extracted from Lemon foliages.

Chapter 5


Finally we can reason the undermentioned tax write-offs from the above experimental and analysis portion.

Volume of Essential Oil additions with addition in temperature by maintaining clip of heating as changeless.

Volume of Essential Oil additions with additions in clip of heating maintaining the temperature invariable.

On diminishing the force per unit area we can pull out Essential Oils of different works stuffs at comparatively less temperature and within less clip of warming.

Major constituents per centum of peculiar Essential Oil will be more if the clip of warming is more at a peculiar temperature.

Analysis utilizing Gas Chromatography-Mass Spectrometer was found to be the best method to place even the minor constituents peculiar oil along with major constituents.

Chapter 6

Cite this Extraction Of Essential Oil Using Steam Distillation Biology

Extraction Of Essential Oil Using Steam Distillation Biology. (2017, Jul 20). Retrieved from https://graduateway.com/extraction-of-essential-oil-using-steam-distillation-biology-essay/

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