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Investigatory Project “ Kaymito Leaves Decoction as Antiseptic Mouthwash ”

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INVESTIGATORY PROJECT “ KAYMITO LEAVES DECOCTION AS ANTISEPTIC MOUTHWASH ” Submitted by: Ronnel S. Pinote I- INTRODUCTION A. Background of the study As an effective mouthwash is one that does not only make the breath fresh but also serves as an antiseptic. It should not simply mask the effective mouth odor caused by excessive activity of anaerobic bacteria inhabiting the mouth. There is a need to prevent the increase in the population. B. Statement of the problem A mouthwash is a solution in addition to regular oral hygiene methods such as brushing and flossing.

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Alternative mouth gargles can be prepared from locally available plants such as herbs and fruit bearing ones. Certain plant parts may have active ingredients that have antimicrobial activity. An example is the peppermint that contains essential oils found to be efficient in neutralizing mouth conditions and in freshening breath. This study Aims to answer following questions: • Is Kaymito leaves decoction can be effective mouthwash? • Is the peppermint that contains essential oils can be effective in neutralizing mouth conditions and in freshening breath? C.

Significance of the study

In this study the people will benefit because they can use it in the prevention of dental diseases and maintenance of the oral health and it can be used for preventing gums and oral infections. D. Scope and Delimitation of the study This study only tested the feasibility of using decoction prepared from kaymito leaves as mouthwash II- REVIEW OF RELATED LITERATURE A. Review of Literature Kaymito (chrisophyllum cainito) is a common angiosperm in tropical countries. This fruit-bearing tree has leathery oblong leaves that are dark green and glossy on the upper surface and golden brown on the underside, its fleshy fruits are among the favorites.

To treat swollen gums, De Guzman-Ladion (1995) suggests to a gargle concoction from its leaves boiled with bayabas (guava), duhat(berry) and talong (eggplant) leaves . Peppermint (Mentha cordifolia) is the most important commercial species of the mint family. The leaves contain oils that are distilled and used as flavoring in chewing gums, toothpaste, candy, and medicines. The oils are reported to cause the cool aftertaste and are used as raw materials for dentifrices, soaps, and perfumes. B. Conceptual framework = C. Hypothesis I think that Kaymito as an antiseptic mouthwash will be effective.

D. Definition of terms • Mouthwash- is a solution used in addition to regular oral hygiene methods such as brushing and flossing • Kaymito- (in English: Starapple, scientific name Chrisophyllum cainito) is a common angiosperm in tropical countries. This fruit bearing tree has leathery oblong leaves that are dark green and glossy on the upper surface and golden brown on the underside, • Peppermint- (scientific name: mentha cordifolia) is the most important commercial species of the mint family. • Decoction- preparation made by boiling herbal matter in water for a period of time. Diffusion- the flow of energy or matter from a higher concentration to a lower concentration, resulting in a homogeneous distribution. III- METHODOLOGY A. EXPERIMENTAL VARIABLE CONTROLLED VARIABLE B. INDEPENDENT VARIABLE DEPENDENT VARIABLE C. – The data was gathered by conducting an experiment. D. Four treatments were prepared and tested for their inhibitory effect against reproduction and colony growth of mouth bacteria. Treatment # 1 – boiling cups of ground, fresh kaimito leaves in five cups of distilled water. Treatment # 2 – 2 ? cups of kaymito leaves in five cups of distilled water.

Treatment # 3 (which serve as a positive control, a proven herbal gargle from peppermint leaves) – Adding five teaspoon of peppermint leaf extract into five cups of boiling water Treatment # 4 (which serve as a negative control) – distilled water Procedure: The substrate for bacterial growth was prepared by dissolving nutrient agar into distilled water over medium heat. The solidified agar media were poured into petri plates. Each petri plate was inoculated with diluted bacteria from saliva samples were used for diffusion method in which the antimicrobial activity of the treatments prepared was tested.

In the said method filter discs were then placed on the surface of the agar medium in plates. There were eight replications per treatment testing. The plates were incubated for 48 hours to give way for bacterial reproduction and colony formation. During this period, the treatments were expected to diffuse from the filter discs into the agar. Among the areas were treatments diffused, bacterial activity was expected to be inhibited as an indication of antimicrobial activity. A zone inhibition was thus tested. IV- RESULTS AND FINDINGS A. | | |TREATMENTS |MEAN VALUE | |Treatment # 1 |4. 125 mm | |Treatment # 2 |2. 125 mm | |Treatment # 3 |4. 5 mm | |Treatment # 4 |-none- | B. The highest mean result (4. 25 mm) was obtained by disc group in treatment # 3 the positive control. This was followed by the computed mean (4. 125) for observations in the plates treated in treatment 1. Based on the paired t-test results the zones of inhibition promoted by the said treatments were significantly different from those observed in the plates treated with treatment # 2, which has a mean value of 2. 25. The filter discs soaked in treatment # 4 did not show any zone of inhibition. V. SUMMARY, CONCLUSIONS AND RECCOMENDATIONS A. Summary The study proved the efficacy of the decoction from the leaves of kaymito (star apple) as an antiseptic mouthwash. Two concentrations of the decoction were tested on the experimental group. The control groups, on the other hand, were applied with peppermint solution, a proven gargle, and distilled water. The antimicrobial property of the four treatments was tested on bacteria cultures.

The parameter used was the zone of inhibition promoted by each treatment. It was found out that the decoction prepared from five cups of kaymito leaves and five cups water showed significant inhibitory effect on the reproduction and colony formation of bacteria. B. Conclusions Based on the results gathered it was concluded that the decoction prepared from five cups of kaymito leaves boiled in five cups of water is as effective as the proven gargle made out of peppermint extract, therefore it can be used as an alternative mouthwash.

C. Recommendations – The group recommends that the people will use mouthwash made from kaimito leaves. ABSTRACT: The study proved the efficacy of the decoction from the leaves of kaymito (star apple) as an antiseptic mouthwash. Two concentrations of the decoction were tested on the experimental group. The control groups, on the other hand, were applied with peppermint solution, a proven gargle, and distilled water. The antimicrobial property of the four treatments was tested on bacteria cultures.

The parameter used was the zone of inhibition promoted by each treatment. It was found out that the decoction prepared from five cups of kaymito leaves and five cups water showed significant inhibitory effect on the reproduction and colony formation of bacteria. ———————– KAIM ITO LEAVES PEPPERMINT By the process of: DECOCTION AND DIFFUSION ANTISEPTIC MOUTHWASH + TWO CONCENTRATIONS OF THE DECOCTION APPLIED WITH PEPPERMINT SOLUTION A PROVEN GARGLE AND DISTILLED WATER MOUTH BACTERIA KAYMITO LEAVES

Cite this Investigatory Project “ Kaymito Leaves Decoction as Antiseptic Mouthwash ”

Investigatory Project “ Kaymito Leaves Decoction as Antiseptic Mouthwash ”. (2018, May 20). Retrieved from https://graduateway.com/investigatory-project-kaymito-leaves-decoction-as-antiseptic-mouthwash-essay/

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