What Factors Affect the Rate of Photosynthesis in Living Leaves? Sample

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In this lab. four different types of foliages were tested to see the rate at which each foliage photosynthesized. This lab demonstrates how workss store light. gaining control visible radiation. and utilize visible radiation as energy for reproduction and growing. by photosynthesis. The control in this experiment was Spinacia oleracea. which was tested before any of the other workss were. The other workss that were tested were English Ivy. C4 Plant. and a motley works. Each type of foliage was tested in a Na hydrogen carbonate solution and a solution of distilled H2O. It will be shown in the treatment whether the hypothesis made was right or wrong. It will be shown in the treatment what could hold occurred during the experiment that could hold affected the consequences. Introduction:

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Photosynthesis can be defined as the procedure by which green workss. and other beings make saccharides from C dioxide and H2O in the presence of chlorophyll. utilizing energy captured from sunlight by chlorophyll. and let go ofing extra O. Like all enzyme-driven reactions. the rate at which photosynthesis occurs can be measured by the disappearing of a certain substrate. or the accretion of merchandise. Photosynthesis consists of light reactions and dark reactions. This procedure can be simplified by the equation: 6CO2 + 6H2O + Energy – & gt ; C6H12O6 + 6O2

Shown in this equation 6 molecules of C dioxide ( 6CO2 ) and 12 molecules of H2O ( 12H2O ) being consumed in the procedure. and glucose ( C6H12O6 ) . six molecules of O ( 6O2 ) . and six molecules of H2O ( 6H2O ) being produced. The energy beginning is photons ( visible radiation ) from the Sun. The rate of photosynthesis could be measured by the sum of moles of O2 produced for one mole of sugar. The rate of photosynthesis could besides be measured by the sum of moles of CO2 that are consumed for every mole of sugar that is synthesized. Leaf discs float in H2O. most of the clip. If air infinites are infused with CO2. nevertheless. the denseness of the discs will increase. When Na hydrogen carbonate is added to H2O. the hydrogen carbonate will do the discs to drop. As photosynthesis continues. O is released into the foliage discs. altering the ability of the foliage discs to drift in H2O. and doing them to lift.

Since cellular respiration is taking topographic point at the same clip inside of the foliage discs. the O generated by photosynthesis will be consumed. As a consequence. the rate at which the discs will lift is relative to the net rate of photosynthesis. In this experiment. the rates of photosynthesis of assorted works foliages were tested. The works used as the control in this experiment was Spinacia oleracea. The Spinacia oleracea foliage was put under the influence of an intense visible radiation beginning and tested in distilled H2O. every bit good as Na hydrogen carbonate. The other foliages that were tested were: C4 works. English Ivy. and a Multicolored Leaf. All three of these diverse workss were tested under the same conditions as the Spinacia oleracea foliage. The hypothesis given for this lab is that the foliage disc will all float to the top of both solutions within 15 proceedingss. Hypothesis the foliage discs will all have risen to the top of the solution within 10-15 proceedingss. except the discs in pure H2O.


* Timer

* 60 watt visible radiation bulb

* Hole cowboy

* Baking sodium carbonate ( sodium hydrogen carbonate )

* Liquid soap ( about 5 milliliters of dishwashing soap in 250 milliliter of H2O )

* 2 plastic panpipes without needle ( 10 milliliter or larger )

* Plant leaves ( Spinacia oleracea. Hedera helix. C4 works. and multicolored plant. )

* 2 clear plastic cup


1. Fix 300 milliliter of 0. 2 % bicarbonate solution for each experiment. ( The hydrogen carbonate will function as a beginning of C dioxide for the foliage discs while they are in the solution. )

2. Label the first cup “CO2”

3. Pour the bicarbonate solution into a clear plastic cup to a deepness of about 3 centimeter

4. Label the 2nd cup “No CO2”5. Fill a 2nd cup with lone H2O.

5.this is to be used as the control group for this experiment

6. Fix the stuffs for both cups at the same time so that the consequences are non skewed

7. Add one bead of a dilute liquid soap solution to each cup utilizing a pipette. If there are bubbles present. do certain to acquire rid of them before go oning the experiment. The soap in this instance is used to wet the hydrophobic surface of the foliage. leting the solution to be drawn into the foliage and enabling the foliage discs to drop in to the fluid.

8. Use a clasp cowboy to cut 10 leaf discs for each cup. Avoid clasp pluging around the ribs of the foliage.

9. Pull the gases out of the squashy mesophyll tissue and infiltrate the foliages with the Na hydrogen carbonate solution. This should be done as follows.

10. Topographic point 5 of the foliage discs into each syringe

11. Put the speculator back in to the syringe. without oppressing the discs. Push in the speculator until merely a little volume of air along with the foliage discs remain in the syringe

12. Put a little volume ( around 5 milliliter ) of Na hydrogen carbonate with the soap solution from the prepared cup into one syringe and a little volume of H2O with soap into the other syringe.

13. Tap each syringe. Assure that the discs are in the solution. after the speculator is inverted. Besides. assure that there is no air staying.Make certain no air remains.

14. At this point. a vacuity is to be created within the syringe to pull the air out of the tissue of the foliage discs. After this measure. the experiment is truly rather simple.

15. To make the vacuity. keep one finger over the narrow portion of the syringe while pulling back the speculator.

16. While keeping the vacuity. twirl the foliage discs to suspend them in the solution.

17. Let go of the vacuity. The solution will infiltrate the air infinites in the foliage discs. which will do them to drop. This process may hold to be repeated a twosome of times if it does non work the first clip.

18. Pour the solution. along with the discs. from the syringe into the appropriate clear plastic cup.

19. Put both cups under the light beginning and get down the timer. At the terminal of each minute. enter the figure of discs that have risen. if any. Assure that no discs are stuck to the side of the syringe.

20. Report observations. and repeat procedure until all of the types of foliages have been tested.

ConsequencesShown in the undermentioned charts is the figure of discs drifting in each solution along with the clip that it took for each disc to lift. The light sours used was placed 29. 5 cm off from the cups in which the discs were placed in. Spinach leaf discs in Sodium Bicarbonate

Number of discs floating| 1| 2| 3| 4| 5|Minutes| 1:57| 5:58| 6:14| 10:02| 18:33|The Spinacia oleracea foliage in this experiment was used as the control. All other informations was compared to that of the Spinacia oleracea foliage discs.

Spinach foliage discs in distilled H2ONumber of discs floating| 1| 2| 3| 4| 5|Minutes| 0:00| 0:00| 0:00| 0:00| 0:00|When the Spinacia oleracea foliage discs were dislodged in to the distilled H2O. none of them rose at any given clip. For this ground. “0:00” was put in to the clip row.

English Ivy in Sodium BicarbonateNumber of discs floating| 1| 2| 3| 4| 5|Minutes| 1:13| 5:07| 12:09| 15:13| 21:07|All 10 discs had floated by 21 proceedingss and 7 seconds.

English Ivy in distilled H2ONumber of discs floating| 1| 2| 3| 4| 5|Minutes| 17:09| 18:31| 19:16| 21:48| 28:14|The English Ivy foliage discs took a longer clip to lift. nevertheless they did stop up lifting towards the terminal of their timing.

Multicolor Plant in Sodium BicarbonateNumber of discs floating| 1| 2| 3| 4| 5|Minutes| 1:04| 11:43| 19:12| 26:05| 32:11|

Multicolor Plant in distilled H2ONumber of discs floating| 1| 2| 3| 4| 5|Minutes| 1:08| 22:16| 23:52| 27:37| 33:02|

C4 works in Sodium BicarbonateNumber of discs floating| 1| 2| 3| 4| 5|Minutes| 1:12| 1:26| 1:28| 7:09| 14:11|The discs of the C4 works rose instead rapidly. much faster than the other workss did in the hydrogen carbonate.

C4 Plant in distilled H2ONumber of discs floating| 1| 2| 3| 4| 5|Minutes| 14:38| 23:04| 26:48| 30:52| 34:45|


The hypothesis made at the beginning of the experiment proved to be incorrect. It was hypothesized that all of the discs would hold risen by 10-15 proceedingss. which was non the instance. Besides. it was predicted that the workss submerged in to the distilled H2O would hold no consequences. which was besides proven incorrect. All but one of the workss that were put in to H2O had shown consequences. Likewise. all but the C4 works in Sodium Bicarbonate solution had taken over 15 proceedingss to hold all of the foliages float to the top of their solution. It was proven in this experiment that the rate of photosynthesis can be affected by the type of works that is being tested on. For illustration. the Ivy works in H2O and the motley works in H2O had wholly different consequences. It took a small over 1 minute for a disc to lift in the motley works. while it took around 17 proceedingss for a individual disc to lift in the Ivy works. Many mistakes could hold occurred in this experiment. Precise timing was important. and if the discs were non submerged in to their solution at the same time. the consequences could hold come out inaccurate. There were issues sing the Spinacia oleracea foliage discs. which could hold been the ground why the 1s that were put in to H2O showed no consequences.

DecisionPhotosynthesis and the rate at which it occurs was shown in this experiment. The different consequences from each type of foliage in the Bicarbonate solution and H2O shows how photosynthesis can be affected by different types of workss and the substance they are soaked in. The hypothesis posed before the experiment had taken topographic point was proven incorrect.


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What Factors Affect the Rate of Photosynthesis in Living Leaves? Sample. (2017, Jul 30). Retrieved from


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