We use cookies to give you the best experience possible. By continuing we’ll assume you’re on board with our cookie policy

See Pricing

What's Your Topic?

Hire a Professional Writer Now

The input space is limited by 250 symbols

What's Your Deadline?

Choose 3 Hours or More.
Back
2/4 steps

How Many Pages?

Back
3/4 steps

Sign Up and See Pricing

"You must agree to out terms of services and privacy policy"
Back
Get Offer

An AP Biology Lab: Inhibiting the Action of Catechol Oxidase

Hire a Professional Writer Now

The input space is limited by 250 symbols

Deadline:2 days left
"You must agree to out terms of services and privacy policy"
Write my paper

This inhibition can be reversed, but only if the substrate’s concentration levels are increasing raised while the inhibitor’s concentration levels are remained stable. Or is PIPIT a noncompetitive inhibitor? A noncompetitive inhibitor also Joins with the enzyme, but it Joins to a site other than the active site. This action changes the enzyme’s nature causing the enzyme to lose its catalytic properties. During this inhibition, the substrate cannot reverse the inhibitor’s actions, so increasing the incineration of the substrate is fruitless.

Don't use plagiarized sources. Get Your Custom Essay on
An AP Biology Lab: Inhibiting the Action of Catechol Oxidase
Just from $13,9/Page
Get custom paper

PIPIT, an inhibitor of cathode oxides, must Join with copper, as a cofactor, in order for the cathode oxides to be active, and PIPIT is known for doing Just that. Group Three hypothesized that PIPIT is in fact a noncompetitive inhibitor based on their knowledge of the nature of competitive and noncompetitive inhibitors. The Group then went on to predict that there won’t be a reaction when the substrate’s concentration is raised because the Group predicted that it is a noncompetitive inhibitor.

Materials and Methods: ? Test tube rack ? 3 small test tubes a RA De 5-ml pipette ? Distilled water ?

Potato extract ? Cathode ? Disposable Pasteur pipettes ? Pipette bulb ? Phenolphthalein (PUT) ? Optional disposable gloves Using Table 3. 1 prepare three test tubes. Add the solutions: water, potato extract, PIPIT, in the order the table suggests, from left to right. Table 3. 1 Contents of the Three Experimental Tubes Debilities Waterproof Extraterrestrial Watercolors 1 5 mama drops drops drops drops 25 mama drops drops 20 drops drops Results: Group Three’s results are depicted by the following table, Table 3. . The first two tubes did not react at all, and the third tube turned brown. Table 3. 2 Results of Inhibition Experiment Deprecation turned Brown Discussion: In this lab, test tubes 1 and 3 were the constant tubes. Tube three was not, and therefore the catechist’s concentration was increased to confirm or refute Group Three’s hypothesis. The substrate, added last, was done so because the substrate loud have initiated the enzyme’s reaction instantly if the inhibitor was not previously there to hinder its reaction, which in this case, it was present.

And, considering the groups needed to see whether or not the inhibitor was competitive or noncompetitive, that wouldn’t have been beneficial. So, is PIPIT a competitive inhibitor or a noncompetitive inhibitor? Group three hypothesized that it is a noncompetitive inhibitor, and went on to predict that there would no reaction after an increase in the substrate’s concentration. Using the data from Table 3. , Table 3. , and knowledge of competitive and noncompetitive inhibitors, Group Three deduced that PIPIT is, in fact, a noncompetitive inhibitor. So, Group Three’s hypothesis was supported, and since there was no reaction after the substrate’s concentration was raised, the prediction was confirmed, too. There are no errors that could have affected Group Three’s results to be documented besides general lab procedure errors. References: 1 . Giles Morgan, J. And Brown Carter, M. E. Investigating Biology 2nd De. Menlo Park, CA: Benjamin/Cummings, 1996.

Cite this An AP Biology Lab: Inhibiting the Action of Catechol Oxidase

An AP Biology Lab: Inhibiting the Action of Catechol Oxidase. (2017, Oct 10). Retrieved from https://graduateway.com/an-ap-biology-lab-inhibiting-the-action-of-catechol-oxidase/

Show less
  • Use multiple resourses when assembling your essay
  • Get help form professional writers when not sure you can do it yourself
  • Use Plagiarism Checker to double check your essay
  • Do not copy and paste free to download essays
Get plagiarism free essay

Search for essay samples now

Haven't found the Essay You Want?

Get my paper now

For Only $13.90/page