The solvents used for the extraction of plant roots were n- butane, ethyl acetate and distilled water. The inviter antibacterial activity was performed by agar well diffusion method. The most susceptible Gram-Positive bacteria was Bacillus SP,
Bureaucratic SP, and the most susceptible Gram-negative bacteria was Brotherhood SP, Calibrated SP, and Incorrectly SP. The extracts of plant Cardiovascular holistically and Milometer heterosexual inhibited the growth of the bacterial strains investigated. The most active extracts was compared with the standard antibiotics, penicillin, Streptomycin and Inclining MGM/disc). The results obtained in the present study suggest that Cardiovascular holistically and Milometer heterosexual could be used in treating diseases caused by the test organisms. The results are discussed in detail.
KEYWORDS: Medicinal Plants, antibacterial activity, aqueous extract, n- butane excretory ethylated extract Medicinal plants are a source of great economic value in the Indian subcontinent. Nature has bestowed on us a very rich botanical wealth and a large number of diverse types of plants grow in different parts of the country. India is rich in all the three levels of biodiversity, namely species diversity, genetic diversity and habitat diversity. In India thousands of species are known to have medicinal value and the use of different parts of several medicinal plants to cure specific aliments has been in vogue since ancient times.
Herbal medicine is still the mainstay of about 75-80% of the whole population, mainly in developing countries. For primary health care because of better cultural acceptability better compatibility with the human body and fewer side effects. However, the last few years have seen a major increase in their use in the developed world . INTRODUCTION: Received on 11. 10. 2011 Accepted on 15. 11. 2011 C Asian Pharmacy press All Right Reserved Asian J. Pram. Rest. 1(4): Cot. – DCE. 2011; Page 111-1 13 Infectious diseases are the leading cause of death world – wide. Antibiotic resistance has become a global concerns.
The Clinical efficacy of many existing antibiotics is being threatened by the emergence of multitude resistant pathogens. Many infection diseases have been known to be treated with herbal remedies throughout the history of mankind. Natural products, either as pure compounds or as standardized plant extract, provide unlimited opportunities for new drug leads because of the unmatched availability of chemical diversity. There is a continuous and urgent need to discover new antimicrobial compounds with diverse chemical structures and novel mechanisms of action for new and re-emerging infectious diseases
Therefore researchers are increasingly turning their attention to folk medicine, looking for new leads to develop better drugs against microbial infections. The increasing failure of chemotherapeutics and antibiotic resistance exhibited by pathogenic microbial infectious agents has led to the screening of several medicinal plants for their potential antimicrobial activity,7. India is a varietals emporium of medicinal plants and is one of the richest countries in the 111 Asian J. Pram. Rest. 201 1; Volvo. 1. Issue 4, Peggy-113 world with regard to genetic resources of medicinal plants.
It exhibits a wide angel in topography and climate, which has a bearing on its vegetation and florist composition moreover, the agro-climatic conditions are conducive for introducing and domesticating new exotic plant varieties PLANT MATERIALS COLLECTION: The plant species namely Cardiovascular, holistically and Milometer heterosexual were collected in Thankful – scripturally cotta. The collected samples were carefully stored in sterile polythene bags and used for the present study. Sterilization of plant materials: About Lugar of fresh and healthy roots was taken for each solvent including aqueous.
The tots of both plants (Cardiovascular holistically and Milometer heterosexual ) were sterilized with running tap water and soaked in 0. 1% mercuric chloride F-anally, the roots were washed with distilled water ( three times ) and shade dried. Composition of nutrient Agar medium: Chemicals Composition Beef extract g Potent g Sodium chloride g egg Agar Distilled water loom PH 7. 0 Antibacterial activity of Cardiovascular holistically: The ethylated extract of Cardiovascular holistically, exhibited maximum zone of inhibition against Bacillus (1 mm), Brotherhood (1 mm), Calibrated (mm),
Incorrectly (mm) and Bureaucratic (mm) were observed. The n- butane extract of Cardiovascular holistically, showed maximum zone of inhibition against Bacillus (mm), Brotherhood (mm), Calibrated (mm), Incorrectly (mm) and Bureaucratic (mm). The aqueous extract of root showed the inhibition zone diameter ranging from 13-mm against tested bacterial pathogens (TABLE-2). Antibacterial activity of Milometer heterosexual: The ethylated extract of Milometer heterosexual exhibited maximum zone of inhibition against Bacillus (mm), Incorrectly (1 Mom) was observed.
The n-butane extract of Milometer heterosexual showed maximum zone of inhibition against Bacillus (mm), Brotherhood (mm), Calibrated (mm), Uncelebrated (mm) and Bureaucratic (mm). The aqueous extract of root showed no zone inhibition against tested bacterial pathogens (TABLE-2). Antibiotic sensitivity test (positive control ): The antibiotic sensitivity test using standard antibiotics, biz. V, streptomycin, inclining and penicillin were tested Preparation of plant root extract: About one gram of sterilized roots were ground in mortar against both bacteria studied.
The results of antibiotic and pestle with 1 Mol of aqueous and organic solvents (ethyl sensitivity represented (TABLE-3). Acetate, and n-butane it was filtered through Whitman Noel filter paper, the supernatant was collected and stored All the antibiotics used were exhibited higher antibacterial for antibacterial screening activity. The results confirmed that both the solvent extract Cardiovascular holistically and Milometer of heterosexual exhibited a higher antibacterial activity Antibacterial activity (Agar -well diffusion method ): The antibacterial activities of the roots were tested against against
Bacillus,Brotherhood, Calibrated, Incorrectly the selected bacterial strains. The ml of sterilized agar and Bureaucratic. Medium was poured into each sterile pipettes and. , allowed to solidify. The test bacterial cultures were evenly Similarly. When compared to the standard antibiotics. The spread over the appropriate media by using sterile cotton, solvent extract of Cardiovascular holistically and Swab. Then a well of 0. CM was made in the medium by Milometer heterosexual showed higher antibacterial activity using a sterile cork borer, 150 Pl of each ethylated, n- against the bacteria TABLE-3). Butane and aqueous plant extracts were transferred into separated wells. After these plates was incubated at ICC Antibacterial effect of solvents (negative control): for 24-48 hours.