Microbiology week

Table of Content

Which of the following stains is used frequently to identify Mycobacterium and other bacteria whose cell walls contain high amounts of lipids? Gram stain Schaeffer-Fulton stain Acid-fast stain Lipid stain Spore Stain 2. Which of the following stains is used to classify microorganisms based on their cell wall content?

Capsular stain Gram stain Spore stain Negative stain Methyl blue 3. In the Gram stain, Gram-positive bacteria stain with a color due to the incorporation of the purple, crystal violet red, seafaring red, crystal violet dye into their cell wall. Purple, seafaring 4. What is the function of the mordant iodine in a Gram staining procedure? A mordant causes the crystal violet to run. A mordant fixes the bacteria to the slide. A mordant keeps dye attached to an object. All of the above 5.

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What is the order of reagents used in the Gram stain? Crystal violet, iodine, seafaring, alcohol alcohol, crystal violet, iodine, seafaring iodine, crystal violet, seafaring, alcohol rascal violet, iodine, alcohol, seafaring crystal violet, seafaring, alcohol, iodine 6. The presence of a capsule around bacterial cells usually indicates their increased disease-causing potential and resistance to disinfection. Capsules are generally viewed by spore staining. Scanning electron microscopy. Gram staining Section: True or False 7.

Fluorescent microscopes use an ultraviolet (IV) light source and fluoroscopes as stains. True False 8. Older, pure cultures of Gram-positive organisms can appear red when Gram stained. True Section: Matching 9. Which microscope is used to view internal structures of unstained, living cells? Scanning electron microscope Dark-field microscopy Transmission electron microscope Phase contrast microscopy Bright-field microscopy Fluorescent Microscope 10. A technique for observing flagella by coating the surfaces of flagella with a dye or a metal such as silver.

Differential stain Flagella stain Schaeffer-Fulton spore stain Simple stain Zilch-Nielsen acid-fast stain Cationic dye (also called basic dye) 1 1 . The Compound light microscope can be used to observe: Ribosome, bacteria, viruses and red blood cells. Yeast, amino acids, bacteria and red blood cells Red blood cells, bacteria and viruses Cell organelles, bacteria and red blood cells Proteins, viruses, atoms and bacteria Bacteria, red blood cells and amino acids 12.

A differential stain used to make endosperm easier to visualize. Differential stain 3 A single dye used to reveal basic cell shapes and arrangements . Differential stain 14. All of the following are parts of a compound microscope except: Condenser Mechanical stage Laser light source Diaphragm Ocular lens Body tube Adjustment knobs 15. An ionic compound, used for staining bacteria, in which the positive ion imparts the color. Differential stain Top of Form Bottom of Form

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