Lactic acid is a hydroxycarboxylic acid, which is widely used in nutrient, pharmaceutical, leather, decorative and textile industries. It can be polymerized to biodegradable and biocompatible plastic, i.e. polylactic acid, which has environment-friendly and great potency for replacing petrochemical plastic. Industrially, it can be produced by either chemical synthesis or microbic agitation. Soon, about 90 % of lactic acid was produced by lactic acid bacteriums agitation.
Fermentative production has the advantage that by taking a strain of LA bacteriums bring forthing merely one of the isomer and optically pure merchandise. The widely used substrates for lactic acid production are pure sugar, which are expensive. It is besides possible to utilize lignocellulosic biomass, particularly by merchandise or waste stuffs from agribusiness and industrial waste as substrates for agitation. Therefore, the usage of alternate substrates, low-cost and natural stuffs become therefore of particular involvement for lactic acid production. The present survey the use of industrial wastes and lignocellulosic as an alternate substrate for lactic acid production.
Lactic acid can be produced by chemical synthesis or microbic agitation. The chemical synthesis produced a racemic mixture of D, L lactic acid. Soon, approximately 90 % of lactic acid made by LAB agitation and the balance is produced synthetically by the hydrolysis of lactonitrile. The advantage of agitation engineerings is possible to utilize renewable resources as substrates, such as amylum and cellulose in fermentative production. In figure 2 described the diagram of commercial utilizations and applications of lactic acid. Food applications reported for about 85 % of the entire lactic acid, while the nonfood industrial applications reported for merely 15 % of the demand.
In agitation procedure, lactic acid bacteriums ( LAB ) need C beginning, basically simple sugars and N beginning, as critical foods for their growings. The widely used substrates for lactic acid production are refined sugar, which are expensive. Therefore, attending has turned towards lignocellulosic biomass and industrial wastes to supply a beginning of saccharide for lactic acid production.
Wee and Ryu ( 2009 ) reported the production of lactic acid from lignocellulosic, glucose and lignocellulosic hydrolyzates were used as the C beginning. The concentration of lactic acid decreases with additions in the dilution rate. Generally, the cell concentration obtained from lignocellulosic hydrolyzates media was about 10-15 % lower than observed with glucose media.
The lignocellulosic hydrolyzates have to be detoxified in order to cut down these repressive effects prior to agitation, as some of the byproducts released during the pretreatment ( Mussatto and Roberto, 2004 ) . Ruengruglikit and Hang ( 2003 ) , reported the lactic acid production from lignocellulosic stuffs by R. oryzae. After an enzymatic hydrolysis and 48-h agitation, lactic acid outputs of 0.3 gg-1.
Food wastes, which are supplied at lower costs, high in wet and rich in saccharides. They could be suited alternate substrate for lactic acid production. The nutrient wastes contain polyoses every bit good as assorted oligosaccharides.
Most of the work utilizing amylum, Beta vulgaris molasses and sugar cane as the agitation media for lactic acid production. Recently, Uno ( 2003 ) used grape saccharase to attractive the production of lactic acid using pineapple sirup as substrate. The efficiency of lactic acid production has been an affect from assorted facet and ability to utilize fruit waste.
Idris and Suzana ( 2005 ) reported the liquid Ananas comosus waste, it is executable to utilize produce lactic acid by immobilized L. delbrueckii. The maximal lactic acid can be obtained when parametric quantities initial pH of 6.5, temperature of 37 & A ; deg ; C and Na alginate concentration at 2 % with a bead diameter of 1 millimeters in diameter.
Recycled paper sludge is an industrial waste has high polyoses ( chiefly cellulose ) content. The transition of polyoses on sludge to be broken down into the monomers and released sugars to be fermented to lactic acid. The methods for transition of a polyose into the monomer consist of enzymatic and acerb hydrolysis.
Trade names et Al. ( 2008 ) studied the use of recycled paper sludge as an alternate substrate for lactic acerb merchandise. The maximal production of lactic acid was produced 73 g/L of lactic acid, maximal productiveness of 2.9 g/L/h, with 0.97 g LA per g of saccharides on initial substrate.
Decisions
The widely used substrates for lactic acid production are refined sugar, which are expensive. It is besides possible to utilize lignocellulosic biomass, particularly by merchandise or waste stuffs from agribusiness and industrial waste as substrates for agitation. Therefore, the usage of alternate, low-priced and natural stuffs become therefore of particular involvement for lactic acid production. The ability to use this industrial wastes and lignocellulosic as alternate C beginnings for lactic acid production will assist cut down of environmental pollution job and besides cut down production costs.
LITERATURE CITED
- Idris, A. and W. Suzana. 2006. Consequence of Na alginate concentration, bead diameter, initial pH and temperature on lactic acid production from pineapple waste utilizing immobilized Lactobacillus delbrueckii. Process Biochem. 41: 1117-1123.
- Kim, K.I. , W.K. Kim, D.K. Seo, I.S. Yoo, E.K. Kim and H.H. Yoon. Production of lactic acid from nutrient wastes Appl. Biochem. Biothnol. 101-108: 637-647.
- Trade names, S. , J.A.L. Santos, F.M. G & A ; Iacute ; Rio de Janeiro and J.C. Roseiro. 2008. Lactic acerb production from recycled paper sludge by coincident saccharification and agitation. Biochem. Eng. 41: 210-216.
- Mussatto, S.I. and I.C. Roberto. 2004. Options for detoxification of diluted-acid lignocellulosic hydrolyzates for usage in fermentative procedures. Bioresour. Technol. 1-10.
- Nexant. 2008. Biotech Route to Lactic Acid/ Polylactic Acid. Available Beginning: hypertext transfer protocol: //nexant.ecnext.com/coms2/summary_0255-130_ITM
- Ohkouchi, Y. and Y. Inoue. 2006. Direct production of L ( + ) -lactic acid from amylum and nutrient wastes utilizing Lactobacillus manihotivorans LMG18011. Bioresour. Technol. 97: 1554-1562.
- Ruengruglikit, C. and Y.D. Hang. 2003. L ( + ) lactic acid production from corn cob by Rhizopus oryzae NRRL-395. Lebensm. Wiss. Technol. 36: 573-575.
- Uno, T. , Y. Ozawa, M. Ishikawa, K. Nakanishi and T. Kimura. 2003. Lactic acid production utilizing two nutrient processing wastes, canned pineapple sirup and grape saccharase as substrate and enzyme. Biotechnol. Lett. 25: 573-577.
- VickRoy, T.B. 1985. Lactic acid, pp. 761-776. In: Blanch, H.W. , S. Drew and D.I.C.
- Wang explosive detection systems. Comprehensive Biotechnol. Vol. 3. Pergamon Press, Oxford.
- Wee, Y.J. , J.N. Kim and H.W. Ryu. 2006. Biotechnological production of lactic acid and its recent applications. Food Technol. Biotechnol. 44 ( 2 ) : 163-172.
- Wee, Y.J. and H.W. Ryu. 2009. Lactic acid production by Lactobacillus sp. RKY2 in a cell-recycle uninterrupted agitation utilizing lignocellulosic hydrolyzates as cheap natural stuffs. Bioresource Technol. 100: 4262-4270.
