The Effect of Alcohol and Caffeine Concentrations on Daphnia Magna

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AbstractionThe mark of this experiment was to analyze the effects of intoxicant and caffeine on the bosom, utilizing the Daphnia Magna as the trial topic.

The hypothesis was: Increasing the concentration of caffeine in which the Daphnia Magna is submerged will do its bosom rate to increase, while increasing the concentration of intoxicant in which it is submerged will do its bosom rate to diminish. To prove this hypothesis two Daphnia Magna were exposed to either intoxicant or caffeine and its bosom rate recorded by puting it on a microscope slide and numbering the figure of bosom beats. It was deducted that the intoxicant and caffeine had a really noteworthy consequence on the bosom, with intoxicant decelerating it down and caffeine rushing it up.At the highest concentration of intoxicant, the bosom rate slowed and in bend the higher concentration of caffeine affected the bosom rate by increasing it.

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I feel that the same construct of the consequence of intoxicant and caffeine on Daphnia Magna would be the same construct for its consequence on worlds besides sense intoxicant is a sedative it tends to decelerate down the organic structure as Caffeine is good known for giving our organic structures a encouragement of energy.IntroductionIt was obvious to prove the effects that alcohol and caffeine have on the human bosom. The effects of intoxicant and caffeine were a distinguishable involvement sense they are drugs that are consumed on a big graduated table in society. However, because it is hard to execute experiments of this nature on worlds and acquire accurate consequences, we decided to utilize Daphnia Magna as a replacement for a human.

Though worlds and Daphnia Magna are different beings, intoxicant and caffeine should hold an about indistinguishable consequence on them. The consequence when both beings are exposed to the same sums of intoxicant and caffeine should be more noticeable in Daphnia Magna because they are much smaller, so in bend the chemicals will flux through the Daphnia Magna’s signifier quicker and consequence each cell more.There are many accounts why Daphnia Magna are normally used compared to the usage of a human as trial topic. First, Daphnia Magna are simple beings compared to worlds therefore there are less factors that we have no control over what could probably impact the bosom rate.

Second, the bosom of the Daphnia Magna is easy to see and analyze because of their transparent organic structure signifier.Third, it is logical to utilize a Daphnia Magna because the effects of the drug will be observed much more rapidly, within minutes compared to around half an hr in worlds. Besides, to acquire useable consequences, it would be necessary to provide a human topic with a sensible big sums of intoxicant or caffeine, which wouldn’t be good for short term wellness, every bit good as unscrupulous. The hypothesis under probe was developed because sedatives, like intoxicant tend to diminish the activity of the body’s variety meats, whilst stimulations like caffeine tend to increase such activity.

Methods and MaterialsTwo Daphnia Magna specimen were collected from the home ground container and so placed each in their ain hanging bead slide carefully so they would non skid all over like they would a traditional level slide. This manner one slide would hold one Daphnia Magna proving the changing concentrations of intoxicant and the other Caffeine.In caffeine we readied concentrations of 0.25 % , 0.

05 % , 0.75 % and 1.0 % degrees of concentration. One member held a bottle of distilled H2O to be used in between concentrations.

The H2O allows the Daphnia Magna to rest a minute that manner it doesn’t dice to rapidly from the assorted concentrations.One member will get down the experiment by matching its bosom beats per seconds to let for an mean bosom rate. The group so subtracted the H2O from the slide and replaced it with the fist concentration of caffeine numbering the bosom beat 15 seconds once more so rapidly taking away the caffeine and replacing it back with distilled H2O. We repeat this procedure and roll up the informations until all concentrations have be used and the information compiled.

Alcohol there were 2 % , 4 % , 6 % , 8 % , and 10 % of concentrations. Using a Microscope every bit good as a fresh bottle of H2O to set the Daphnia Magna to the emphasis of the many concentrations makes the experiment a small easier. As we did with the caffeine the group used a fresh Daphnia Magna collected the mean pulse and began the procedure of subjecting the Daphnia Magna to the Alcohol concentrations roll uping the bosom round informations and detecting how it changes with each concentration.ConsequencesConsequences demonstrates the profound consequence the intoxicant and caffeine both have on the bosom.

As shown in figure 1 and 3 the higher the concentration of caffeine the faster the bosom becomes. In figures 2 and 4 Alcohol seems to hold the opposite affect and slows the bosom rate down progressively with each concentration.Within our consequences it can be determined that when worlds consume intoxicant that they will see a awkwardness and frequently a more down feeling. On the impudent side when worlds are exposed to caffeine it can be seen that there is a explosion of energy “Caffeine is a cardinal nervous system stimulation.

Your cardinal nervous system includes your encephalon, spinal cord, and the other nervousnesss in your organic structure. Caffeine’s chief consequence on your organic structure is to do you experience more awake and qui vive for a while” ( FDA, 2007 )Table 1:group informations for Caffeine concentrations

Caffeine Concentrations and how they affect the bosom rate
Concentration Heart Rate 15 sec Heart Rate ( BPM )
0 % 53 216
0.25 % 42 168
0.50 % 39 156

75 %

28 112
1.0 % 31 124

Figure 1:the graph that shows the group mean bosom rates when exposed to the different concentrations of caffeine provided that the Daphnia Magna bosom rate was counted for 15 seconds so calculated to BPMTable 2:Group informations for intoxicant concentrations

Alcohol concentrations and how they affect the bosom rate
Concentration Heart Rate 15 sec Heart Rate ( BPM )
0 % 31 133.33
2 % 27 108
4 % 24 96
6 % 12 68
8 % 19 76
10 % 14 56

Figure 2:the graph that shows the group mean bosom rates when exposed to the different concentrations of intoxicant provided that the Daphnia Magna bosom rate was counted for 15 seconds so calculated to BPM.Table 3:Class information for caffeine concentrations

Water Control AVG 0.

25 % Caf

0.5 % Caf 0.75 % Caf 1.0 % Caf
KR 216 168 156 112 124
g1 45.


56 84 96 88
G2 248 448 452 480 508
Jeep 132 208 164 220 188
G5 133.3 120 112 124 136
Average 154.92 200 193.6 206.



Figure 3:the graph that shows the category mean bosom rates when exposed to the different concentrations of caffeine.Table 4:Class information for Alcohol concentrations

Initials Water Control AVG 2 % Alc 4 % Alc 6 % Alc 8 % Alc 10 % Alc
KR 133.33 108 96 68 76 56
g1 54 248 285 236 188 172
G2 205.


312 240 180 64 48
Jeep 181 148 152 124 124 108
G5 205.3 240 196 232 212 100
Average 155.8 211.2 193.


168 132.8 96.8

Figure 4:the graph that shows the category mean bosom rates when exposed to the different concentrations of intoxicant.Discussion“Alcohol affects every organ in the drinker ‘s organic structure and can damage a development foetus.

Poisoning can impair encephalon map and motor accomplishments ; heavy usage can increase hazard of certain malignant neoplastic diseases, shot, and liver disease.” ( Abuse, 2014 )

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The Effect of Alcohol and Caffeine Concentrations on Daphnia Magna. (2016, Nov 30). Retrieved from

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