Jollily Temperature and enzyme activity Aim: To determine the effect of which the temperature of the enzyme has on the rate of the enzyme catalysts reaction. Hypothesis: The rate of reaction of an enzyme catalysts reaction will increase as the temperature of the enzyme approaches the optimum temperature. Surpassing the optimum temperature will result in a drop in enzyme activity. Materials: 6% hydrogen peroxide Liver suspension 10 test tubes 4 beakers Thermometers Measuring cylinders Test tube pegs Test tube rack Bunsen burner
Tripod Gauze mat Ice cubes/refrigerated water Ruler Safety goggles Pipettes Method: 1 .
Set up equipment as follows: Beaker Temperature ( ; C) 6% Hydrogen peroxide (ml) Liver suspension Fill beaker 1 with mall of ice cubes and tap water and record temperature. 3. Place a pair of test tubes, one of hydrogen peroxide and the other of catalane, into the cold water bath (Beaker 1) for 2 minutes. 4. Remove test tubes from the beaker simultaneously and immediately pour the catalane into the hydrogen peroxide. Measure height of froth immediately.
Repeat steps 2-4 with beaker 2 and tap water. 6. Repeat steps 3 and 4 with beaker 3 with no water, record room temperature. 7. Repeat steps 2-4 with beaker 4 and heated water of 37 ;C. 8. Repeat steps 2-4 with beaker 5 and heated water of 50;C. Risk assessment: As hydrogen peroxide is an irritant, wear safety goggles to protect eyes from possible splashes and clean up any spillages quickly. Equipment will be hot and may cause burns. Handle equipment with caution and use test tube pegs when dealing with hot test tubes. Results: Temperature ( ;C) Reaction rate – Froth height (mm)
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